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Titolo:
Novel pristinamycin-responsive expression systems for plant cells
Autore:
Frey, AD; Rimann, M; Bailey, JE; Kallio, PT; Thompson, CJ; Fussenegger, M;
Indirizzi:
ETH Zurich, Swiss Fed Inst Technol, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8093 chnol, CH-8093 Zurich, Switzerland
Titolo Testata:
BIOTECHNOLOGY AND BIOENGINEERING
fascicolo: 2, volume: 74, anno: 2001,
pagine: 154 - 163
SICI:
0006-3592(20010720)74:2<154:NPESFP>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
TN10-ENCODED TET-REPRESSOR; MULTIDRUG-RESISTANCE GENE; NONSTEROIDAL ECDYSONE AGONIST; TRANSGENIC TOBACCO; STREPTOMYCES-PRISTINAESPIRALIS; REGULATED EXPRESSION; MAMMALIAN-CELLS; PROMOTER; PROTEIN; BINDING;
Keywords:
plant gene regulation; pristinamycin; streptogramin; Pip; tobacco; Streptomyces;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Fussenegger, M ETH Zurich, Swiss Fed Inst Technol, Inst Biotechnol, CH-8093 Zurich, Switzerland ETH Zurich Zurich Switzerland CH-8093 Zurich, Switzerland
Citazione:
A.D. Frey et al., "Novel pristinamycin-responsive expression systems for plant cells", BIOTECH BIO, 74(2), 2001, pp. 154-163

Abstract

Novel gene regulation systems were designed for plant cells responsive to the streptogramin antibiotic pristinamycin. The pristinamycin-repressible plant gene regulation concept (PIPpOFF) is based on a transcriptional activator (PIT) which consists of the Pip protein, the repressor of the pristinamycin resistance operon of Streptomyces coelicolor fused to the VP16 transactivation domain of the Herpes simplex virus. PIT mediates pristinamycin-repressible activation of a synthetic plant promoter (P-pPIR) in tobacco cellsconsisting of a nine Pip-binding site-containing artificial operator (PIR3) placed upstream of a TATA-box derived from the cauliflower mosaic virus 35S promoter (P-CaMV35S). Pristinamycin interferes with induction by negatively regulating the DNA-binding capacity of the Pip moiety of PIT. A second,streptogramin-inducible plant gene regulation system (PIPpON) was constructed by combining Pip expression with a plant-specific pristinamycin-inducible promoter (P-pPIRON). P-pPIRON consists of a PIR3 module cloned downstream of the strong constitutive plant promoter P-CaMV35S. As in the native Streptomyces configuration, Pip binds to its cognate sequence within P-pPIRON in the absence of regulating antibiotic and silences the chimeric plant promoter. Upon addition of pristinamycin, Pip is released from the PIR3 operator and full P-CaMV35S- driven expression of desired plant genes is induced. The PIPpOFF and PIPpON systems performed well in Nicotiana tabacum suspension cultures and promise to provide an attractive extension of existing plant gene regulation technology for basic plant research or biopharmaceuticalmanufacturing using plant tissue culture. (C) John Wiley & Sons, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 14:14:34