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Titolo:
A simple HPLC method to determine urinary phenylmercapturic acid and its application to gasoline station attendants to biomonitor occupational exposure to benzene at less than 1 ppm
Autore:
Inoue, O; Kanno, E; Yusa, T; Kakizaki, M; Watanabe, T; Higashikawa, K; Ikeda, M;
Indirizzi:
Tohoku Rosai Hosp, Sendai, Miyagi 9818563, Japan Tohoku Rosai Hosp SendaiMiyagi Japan 9818563 ndai, Miyagi 9818563, Japan Miyagi Univ Educ, Sendai, Miyagi 9800845, Japan Miyagi Univ Educ Sendai Miyagi Japan 9800845 endai, Miyagi 9800845, Japan
Titolo Testata:
BIOMARKERS
fascicolo: 3, volume: 6, anno: 2001,
pagine: 190 - 203
SICI:
1354-750X(200105/06)6:3<190:ASHMTD>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
GAS-CHROMATOGRAPHIC METHOD; MERCAPTURIC ACIDS; T,T-MUCONIC ACID; CAR MECHANICS; WORKERS; BIOMARKERS; URINALYSIS; INDICATOR; SOLVENTS; PHENOL;
Keywords:
benzene; biological monitoring; gasoline station; occupational exposure; phenylmercapturic acid; urinalysis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Ikeda, M Kyoto Ind Hlth Assoc, Nagakyo Ku, 67 Nishinokyo Kitatsuboicho, Kyoto 6048472, Japan Kyoto Ind Hlth Assoc 67 Nishinokyo Kitatsuboicho Kyoto Japan 6048472
Citazione:
O. Inoue et al., "A simple HPLC method to determine urinary phenylmercapturic acid and its application to gasoline station attendants to biomonitor occupational exposure to benzene at less than 1 ppm", BIOMARKERS, 6(3), 2001, pp. 190-203

Abstract

The objective of this study was to establish a hand-saving method to measure phenylmercapturic acid (PMA) and to examine urinary PMA as a marker of occupational exposure to benzene at levels less than 1 ppm. A simple HPLC method was developed to analyse PMA by monitoring absorption at 195 nm of theeffluent from an ODS-3 column with acetonitrile-methanol-perchloric acid-water as a mobile phase. The detection limit of the method was 0.2 mug l(-1)with sufficient reproducibility. The method was applied to end-of-shift urine samples from 70 gasoline station attendants exposed to up to 107 ppb benzene, and 20 non-exposed controls of both sexes. Time-weighted average (TWA) exposure to benzene was measured by diffusive sampling. A regression analysis was applied to examine the quantitative relationship between the intensity of exposure to benzene and PMA in the end-of-shift urine samples. Multiple regression analysis showed no effects of age, sex, smoking and co-exposure to toluene and xylenes on urinary PMA. There was a linear relationship between TWA benzene exposure and urinary PMA (r = 0.60-0.67, P < 0.01). Background PMA in urine of the non-exposed controls was low and scattering of PMA around the regression line was narrow so that those with 20 ppb benzene exposure can be separated from the non-exposed by urinalysis for PMA. Thus, urinary PMA is sensitive enough for biological exposure monitoring of those exposed to less than 1 ppm benzene.

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Documento generato il 06/04/20 alle ore 22:34:30