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Titolo:
Intracellular ascorbic acid enhances the DNA single-strand breakage and toxicity induced by peroxynitrite in U937 cells
Autore:
Guidarelli, A; De Sanctis, R; Cellini, B; Fiorani, M; Dacha, M; Cantoni, O;
Indirizzi:
Ist Farmacol & Farmacognosia, I-61029 Urbino, Italy Ist Farmacol & Farmacognosia Urbino Italy I-61029 I-61029 Urbino, Italy Univ Urbino, Ist Chim Biol Giorgio Fornaini, I-61029 Urbino, Italy Univ Urbino Urbino Italy I-61029 Giorgio Fornaini, I-61029 Urbino, Italy
Titolo Testata:
BIOCHEMICAL JOURNAL
, volume: 356, anno: 2001,
parte:, 2
pagine: 509 - 513
SICI:
0264-6021(20010601)356:<509:IAAETD>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
NITRIC-OXIDE; VITAMIN-C; CELLULAR FERRITIN; DEHYDROASCORBATE; GLUTATHIONE; SUPEROXIDE; REDUCTION; ANTIOXIDANTS; OXIDATION; ASSAY;
Keywords:
cell death; DNA damage; reactive nitrogen species; vitamin C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Cantoni, O Ist Farmacol & Farmacognosia, Via S Chiara 27, I-61029 Urbino, Italy Ist Farmacol & Farmacognosia Via S Chiara 27 Urbino Italy I-61029
Citazione:
A. Guidarelli et al., "Intracellular ascorbic acid enhances the DNA single-strand breakage and toxicity induced by peroxynitrite in U937 cells", BIOCHEM J, 356, 2001, pp. 509-513

Abstract

A well-established protocol to increase the intracellular content of ascorbic acid was used to investigate the effects of the vitamin on DNA single-strand breakage and toxicity mediated by authentic peroxynitrite (ONOO-) in U937 cells. This protocol involved exposure for 60 min to 100 muM dehydroascorbic acid, which was taken up by the cells and converted into ascorbic acid via a GSH-independent mechanism. At the time of exposure to ONOO-, whichwas performed in fresh saline immediately after loading with dehydroascorbic acid, the vitamin present in the cells was all in its reduced form. It was found that, in cells that are otherwise ascorbate-deficient, an increasein their ascorbic acid content does not prevent, but rather enhances, the DNA-damaging and lethal responses mediated by exogenous ONOO-. These results therefore suggest that acute supplementation of ascorbic acid can be detrimental for individuals with pathologies associated with a decrease in ascorbic acid and in which ONOO- is known to promote deleterious effects.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 25/11/20 alle ore 10:28:14