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Titolo:
Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry
Autore:
Nishigaki, T; Zamudio, FZ; Possani, LD; Darszon, A;
Indirizzi:
UNAM, Inst Biotecnol, Dept Reconocimiento Mol & Biostruct, Cuernavaca 62250, Morelos, Mexico UNAM Cuernavaca Morelos Mexico 62250 t, Cuernavaca 62250, Morelos, Mexico UNAM, Inst Biotecnol, Dept Genet & Fisiol Mol, Cuernavaca 62250, Morelos, Mexico UNAM Cuernavaca Morelos Mexico 62250 l, Cuernavaca 62250, Morelos, Mexico
Titolo Testata:
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
fascicolo: 2, volume: 284, anno: 2001,
pagine: 531 - 535
SICI:
0006-291X(20010608)284:2<531:TSRTAE>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-URCHIN SPERM; ACTIVATING PEPTIDES; JELLY COAT; CHANNEL; SPERMATOZOA; MEMBRANE; IDENTIFICATION; MODULATION; RECEPTOR; RESACT;
Keywords:
egg peptide; sperm activation; stopped-flow; ligand-receptor interaction; fluorescence quenching; intracellular pH; intracellular Ca2+; chemotaxis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Darszon, A UNAM, Inst Biotecnol, Dept Genet & Fisiol Mol, Ave Univ 2001,Colonia Chamilpa, Cuernavaca 62210, Morelos, Mexico UNAM Ave Univ 2001,Colonia Chamilpa Cuernavaca Morelos Mexico 62210
Citazione:
T. Nishigaki et al., "Time-resolved sperm responses to an egg peptide measured by stopped-flow fluorometry", BIOC BIOP R, 284(2), 2001, pp. 531-535

Abstract

Speract, a decapeptide from sea urchin egg jelly, induces various sperm responses. Stopped-flow fluorometry was used to examine the binding of labeled speract and the intracellular changes in pH (pH(i)) and Ca2+ ([Ca2+](i)) it induces in sperm. We observed significant time delays for the increase in pH(i), and [Ca2+](i) induced by 200 nM speract (69 and 190 ms, respectively). Also, we found that the receptor undergoes a pH(i)-dependent affinity change at around 129 ms. These time delays probably reflect biochemical processes underlying each sperm response to speract that circumscribe the timesequence of the signaling events. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 03:50:16