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Titolo:
Endogenous neutralizing antibodies against platelet-derived growth factor-AA inhibit atherogenesis in the cholesterol-fed rabbit
Autore:
Lamb, DJ; Avades, TY; Ferns, GAA;
Indirizzi:
Univ Surrey, Sch Biol Sci, Ctr Clin Sci & Measurement, Guildford GU2 5XH, Surrey, England Univ Surrey Guildford Surrey England GU2 5XH ord GU2 5XH, Surrey, England Royal Surrey Cty Hosp, Dept Clin Biochem, Guildford, Surrey, England RoyalSurrey Cty Hosp Guildford Surrey England uildford, Surrey, England
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 6, volume: 21, anno: 2001,
pagine: 997 - 1003
SICI:
1079-5642(200106)21:6<997:ENAAPG>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN ATHEROSCLEROTIC PLAQUES; GENE-EXPRESSION; CALMETTE-GUERIN; CELLS; PROLIFERATION; PROTEIN; MICE; IMMUNOGLOBULIN; RECEPTORS; LIGAND;
Keywords:
platelet-derived growth factor-AA; endogenous antibody; atherosclerosis; cholesterol-fed rabbit;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Ferns, GAA Univ Surrey, Sch Biol Sci, Ctr Clin Sci & Measurement, Guildford GU2 5XH, Surrey, England Univ Surrey Guildford Surrey England GU2 5XH , Surrey, England
Citazione:
D.J. Lamb et al., "Endogenous neutralizing antibodies against platelet-derived growth factor-AA inhibit atherogenesis in the cholesterol-fed rabbit", ART THROM V, 21(6), 2001, pp. 997-1003

Abstract

Previous studies have shown that the B chain of platelet-derived growth factor (PDGF) has an important role in atherogenesis. In this study we have investigated the contribution of PDGF-A chain in cholesterol-induced atherogenesis in the New Zealand White rabbit. High titers of antibodies to PDGF-AA or to platelet cytosolic protein (PCP) were induced in these animals by immunization against recombinant human PDGF-AA or human PCP. Rabbits were then fed a 0.25% to 1% cholesterol-containing diet for 10 weeks to induce atherosclerotic lesions; the rabbits were then humanely killed and perfusion-fixed and their aortas were removed. The extent of atherosclerosis in the thoracic aortas was determined by quantitative morphometry after staining with oil red O. The intimal and medial areas in histological sections taken atthe level of the first intercostal branch were quantified by image analysis. Immunization against PDGF-AA and PCP, but not against adjuvant alone, resulted in rising titers of antibodies within 2 weeks, the levels of which reached a plateau by 8 weeks. The antibodies to PDGF-AA were isoform-specific, recognized both human and rabbit PDGF-AA, and neutralized the biologicalactivity of PDGF-AA in vitro. Integrated plasma cholesterol levels were similar in both groups. Compared with nonimmune rabbits (n=10), animals immunized against PDGF-AA (n= 10) or PCP (n= 10) had significantly smaller areasof the aorta covered by atherosclerotic lesions (24.6 +/-5.1% and 18.7 +/-4.2%, respectively, vs 34.4 +/-4.3%; P <0.05). This was associated with a reduced aortic intimal-medial area ratio in PDGF-AA-immunized (0.009 +/-0.006) and PCP-immunized (0.025 +/-0.017) rabbits than in nonimmnne animals (0.159 +/-0.066: P <0.05). These data suggest that PDGF-AA is actively involved in cholesterol-induced atherosclerosis in the rabbit.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 11:19:58