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Titolo:
Function of GATA transcription factors in induction of endothelial vascular cell adhesion molecule-1 by tumor necrosis factor-alpha
Autore:
Umetani, M; Mataki, C; Minegishi, N; Yamamoto, M; Hamakubo, T; Kodama, T;
Indirizzi:
Univ Tokyo, RCAST, Dept Mol Biol & Med, Tokyo 1530084, Japan Univ Tokyo Tokyo Japan 1530084 Dept Mol Biol & Med, Tokyo 1530084, Japan Univ Tsukuba, Ctr Tsukuba Adv Res Alliance, Tsukuba, Ibaraki 305, Japan Univ Tsukuba Tsukuba Ibaraki Japan 305 iance, Tsukuba, Ibaraki 305, Japan
Titolo Testata:
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
fascicolo: 6, volume: 21, anno: 2001,
pagine: 917 - 922
SICI:
1079-5642(200106)21:6<917:FOGTFI>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
NF-KAPPA-B; GENE-EXPRESSION; BINDING PROTEIN; UP-REGULATION; PROMOTER; DIFFERENTIATION; ENHANCER; PHOSPHORYLATION; ATHEROSCLEROSIS; HEMATOPOIESIS;
Keywords:
endothelial cells; vascular cell adhesion molecule-1; GATA transcription factor; tumor necrosis factor-alpha; human umbilical vein endothelial cells;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Umetani, M Univ Tokyo, RCAST, Dept Mol Biol & Med, 35,4-6-1 Komaba, Tokyo 1530084, Japan Univ Tokyo 35,4-6-1 Komaba Tokyo Japan 1530084 1530084, Japan
Citazione:
M. Umetani et al., "Function of GATA transcription factors in induction of endothelial vascular cell adhesion molecule-1 by tumor necrosis factor-alpha", ART THROM V, 21(6), 2001, pp. 917-922

Abstract

Endothelial vascular cell adhesion molecule-1 (VCAM-1) is expressed in response to cytokine stimulation and plays a critical role in inflammatory reactions. Previously, we developed a novel VCAM-1 inhibitor that acts througha mechanism independent of nuclear factor-kappaB activity. It suppresses the binding activity of GATA proteins in cytokine-stimulated endothelial cells, which may be related to the anti-VCAM-1 induction effect of this drug. In this study, we investigated the role of GATA proteins in the induction of VCAM-1 by tumor necrosis factor-alpha (TNF-alpha) in human endothelial cells. The mRNA expression of GATA-6 was increased, whereas GATA-3 mRNA was decreased by TNF-alpha stimulation. Electrophoretic mobility shift assay showed that TNF-alpha stimulation increased the DNA binding of GATA-6 but decreased that of GATA-3. Experiments using protein overexpression or antisenseoligonucleotides revealed that GATA-6 potently acts as a positive regulator of VCAM-1 gene transcription. In contrast. overexpression of GATA-3 was able to suppress TNF-alpha -induced VCAM-1 expression. Our results provide evidence of the importance of GATA proteins in the induction of VCAM-1 by TNF-alpha in vascular endothelial cells. The switch from GATA-3 to GATA-6 is taken to be an important transcriptional control event in TNF-alpha induction of VCAM-1.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 10:09:33