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Titolo:
THE 46-KDA MANNOSE 6-PHOSPHATE RECEPTOR CONTAINS MULTIPLE BINDING-SITES FOR CLATHRIN ADAPTERS
Autore:
HONING S; SOSA M; HILLEREHFELD A; VONFIGURA K;
Indirizzi:
UNIV GOTTINGEN,INST BIOCHEM 2,GOSSLERSTR 12D D-37073 GOTTINGEN GERMANY UNIV GOTTINGEN,INST BIOCHEM 2 D-37073 GOTTINGEN GERMANY
Titolo Testata:
The Journal of biological chemistry
fascicolo: 32, volume: 272, anno: 1997,
pagine: 19884 - 19890
SICI:
0021-9258(1997)272:32<19884:T4M6RC>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SURFACE-PLASMON RESONANCE; FACTOR-II RECEPTOR; CYTOPLASMIC DOMAIN; COATED VESICLES; INVITRO BINDING; COMPLEX AP-2; MEMBRANE; SIGNALS; PROTEINS; GOLGI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
43
Recensione:
Indirizzi per estratti:
Citazione:
S. Honing et al., "THE 46-KDA MANNOSE 6-PHOSPHATE RECEPTOR CONTAINS MULTIPLE BINDING-SITES FOR CLATHRIN ADAPTERS", The Journal of biological chemistry, 272(32), 1997, pp. 19884-19890

Abstract

The two known mannose 6-phosphate receptors (MPR46 and MPR300) both mediate the transport of Man-6-P-containing lysosomal proteins to lysosomes. However, the MPRs cannot be detected in lysosomes, instead they recycle between the plasma membrane and endosomes and between endosomes and the trans-Golgi network. Both, endocytosis from the plasma membrane and budding of transport vesicles from the trans-Golgi network involves the interaction of the receptor with the clathrin-coated vesicles-associated protein complexes AP1 and AP2. We have analyzed this interaction between the Golgi-restricted AP1 complex and the plasma membrane-restricted AP2 complex with the MPR46 tail in vitro by using a biosensor. AP1 and AP2 both bind to and dissociate from the MPR46 tail with similar kinetics, Using synthetic peptides corresponding to different MPR receptor tail regions in inhibition and binding studies, a common high affinity binding site for AP1 and AP2 and two separate high affinity binding sites for AP1 and AP2, respectively, were identified.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:01:48