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Titolo:
Tet-system for the regulation of gene expression during embryonic development
Autore:
Fedorov, LM; Tyrsin, OY; Krenn, V; Chernigovskaya, EV; Rapp, UR;
Indirizzi:
Univ Wurzburg, Inst Med Strahlenkunde & Zellforsch, D-97078 Wurzburg, Germany Univ Wurzburg Wurzburg Germany D-97078 forsch, D-97078 Wurzburg, Germany Charite, Inst Pathol, D-10117 Berlin, Germany Charite Berlin Germany D-10117 ite, Inst Pathol, D-10117 Berlin, Germany RAN, IM Sechenov Evolutionary Physiol & Biochem Inst, St Petersburg 194223, Russia RAN St Petersburg Russia 194223 ochem Inst, St Petersburg 194223, Russia
Titolo Testata:
TRANSGENIC RESEARCH
fascicolo: 3, volume: 10, anno: 2001,
pagine: 247 - 258
SICI:
0962-8819(200106)10:3<247:TFTROG>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSGENIC MICE; CONDITIONAL EXPRESSION; TETRACYCLINE; PROMOTER; HYPERPLASIA; ENHANCER; RECEPTOR; MUTANTS; TISSUES; CELLS;
Keywords:
bidirectional promoter; development; doxycycline; transgenic mice; tTA/rtTA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Fedorov, LM Univ Wurzburg, Inst Med Strahlenkunde & Zellforsch, VersbacherStr 5, D-97078 Wurzburg, Germany Univ Wurzburg Versbacher Str 5 Wurzburg Germany D-97078 rmany
Citazione:
L.M. Fedorov et al., "Tet-system for the regulation of gene expression during embryonic development", TRANSGEN RE, 10(3), 2001, pp. 247-258

Abstract

The ability to control gene expression in a temporal and spatial manner provides a new tool for the study of mammalian gene function particularly during development and oncogenesis. In this study the suitability of the tet-system for investigating embryogenesis was tested in detail. The tTA(CMV) (M1) and rTA(CMV)-3 (reverse Tc-controlled transactivator) transgenic mice were bred with NZL-2 bi-reporter mice containing the vector with a tTA/rTA responsive bidirectional promoter that allows simultaneous regulation of expression of two reporter genes encoding luciferase and beta -galactosidase. In both cases reporter genes were found to be expressed in a wide spectrum of tissues of double transgenic embryos and adult mice. The earliest expression was detected in tTA(CMV)(M1)/NZL-2 embryos at embryonic day 10.5 (E10.5) and rTA(CMV)-3/NZL-2 embryos at E13.5. Doxycycline abolished beta -gal expression in tTA(CMV) (M1)/NZL-2 but induced it in rTA(CMV)-3/NZL-2 embryos including late stages of embryogenesis. The tTA and rtTA transactivators thus revealed a partially complementary mode of action during second half of embryonic development. These experiments demonstrated that both Tet regulatory systems function during embryonic development. We conclude that the Tetsystems allows regulation of gene expression during embryonic development and that 'double reporter' animals like the NZL-2 mice are useful tools forthe characterization of newly generated tet transactivator lines expressing tTA (or rtTA) in embryonic as well as in adult tissues.

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Documento generato il 03/04/20 alle ore 19:54:19