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Titolo:
Differential expression of gastrin, cholecystokinin-A and cholecystokinin-B receptor mRNA in human pancreatic cancer cell lines
Autore:
Monstein, HJ; Ohlsson, B; Axelson, J;
Indirizzi:
Malmo Univ Hosp, Dept Surg, MAS, SE-20502 Malmo, Sweden Malmo Univ Hosp Malmo Sweden SE-20502 Surg, MAS, SE-20502 Malmo, Sweden Linkoping Univ Hosp, LMO, Mol Biol Lab, S-58185 Linkoping, Sweden Linkoping Univ Hosp Linkoping Sweden S-58185 , S-58185 Linkoping, Sweden
Titolo Testata:
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
fascicolo: 7, volume: 36, anno: 2001,
pagine: 738 - 743
SICI:
0036-5521(200107)36:7<738:DEOGCA>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
MESSENGER-RNA EXPRESSION; STIMULATES GROWTH; FUNCTIONAL EXPRESSION; MOLECULAR-CLONING; CCK-B; GUINEA-PIG; RAT; ADENOCARCINOMA; PROLIFERATION; PROGASTRIN;
Keywords:
cholecystokinin (CCK); CCK-A receptor; CCK-B receptor; gastrin; gene expression; pancreatic cancer;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Ohlsson, B Malmo Univ Hosp, Dept Surg, MAS, SE-20502 Malmo, Sweden Malmo Univ Hosp Malmo Sweden SE-20502 SE-20502 Malmo, Sweden
Citazione:
H.J. Monstein et al., "Differential expression of gastrin, cholecystokinin-A and cholecystokinin-B receptor mRNA in human pancreatic cancer cell lines", SC J GASTR, 36(7), 2001, pp. 738-743

Abstract

Background: It has been assumed that gastrin stimulates the growth of pancreatic cancer in an autocrine way through co-expression of gastrin and the cholecystokinin-B receptor (CCK-BR). However, pancreatic cancer cell lines established directly from patients have revealed a great heterogeneity in cell proliferation when exposed to CCK, gastrin and their receptor antagonists. The aim of this study was therefore to examine co-expression of CCK-A and CCK-B receptor (CCK-AR and CCK-BR), and gastrin mRNA as well as the secretion of CCK and gastrin peptides in these cell lines. Methods: Fourteen cell lines were established from primary pancreatic cancers or their metastases. Total RNA was isolated from the cell lines and reverse-transcribed intosingle-stranded cDNA. A PCR technique based on Tag polymerase-antibody interaction and CCK-AR, CCK-BR and gastrin-specific primers, followed by Southern blot analysis, were the methods used. The incubation mediums were analysed for the presence of secreted CCK/proCCK and gastrin/progastrin peptidesby specific radioimmunoassays (RIA). Results: By means of nested Reverse-Transcribed Polymerase Chain Reaction (nested RT-PCR), combined with Southern blot analysis of the PCR amplified products, CCK-AR and gastrin mRNA co-expression was detected in cell Lines LPC-6p and LPC-10m, whereas CCK-BR andgastrin mRNA could be detected in cell lines LPC-8p and LPC-12m. A low level of secreted CCK peptides was detected in cell line LPC-6p. which also expressed CCK-AR mRNA. In no other cases were CCK or gastrin peptides detected in the cell culture mediums. Conclusion: The lack of CCK-BR and gastrin mRNA co-expression, and not detectable levels of secreted CCK and gastrin inculture media, does not lend support to the hypothesis that concomitant gene-expression of CCK receptors and gastrin or CCK are essential to maintaining pancreatic cancer cell proliferation.

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Documento generato il 28/09/20 alle ore 18:03:27