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Titolo:
Antisecretory effect of loperamide in colon epithelial cells by inhibitionof basolateral K+ conductance
Autore:
Epple, HJ; Fromm, M; Riecken, EO; Schulzke, JD;
Indirizzi:
Free Univ Berlin, Klinikum Benjamin Franklin, Med Gastroenterol & Infekt Klin 1, Dept Gastroenterol, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 enterol, D-12200 Berlin, Germany Free Univ Berlin, Klinikum Benjamin Franklin, Dept Clin Physiol, D-12200 Berlin, Germany Free Univ Berlin Berlin Germany D-12200 Physiol, D-12200 Berlin, Germany
Titolo Testata:
SCANDINAVIAN JOURNAL OF GASTROENTEROLOGY
fascicolo: 7, volume: 36, anno: 2001,
pagine: 731 - 737
SICI:
0036-5521(200107)36:7<731:AEOLIC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; OPIOID RECEPTORS; RABBIT COLON; SECRETION; INVITRO; LINE; MECHANISMS; MONOLAYERS; CALMODULIN; TRANSPORT;
Keywords:
calcium; calmodulin; cyclic AMP; chloride secretion; diarrhoea; HT-29; potassium conductance; potassium channel; protein kinase C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
34
Recensione:
Indirizzi per estratti:
Indirizzo: Schulzke, JD Free Univ Berlin, Klinikum Benjamin Franklin, Med Gastroenterol & Infekt Klin 1, Dept Gastroenterol, Hindenburgdamm 30, D-12200 Berlin, Germany Free Univ Berlin Hindenburgdamm 30 Berlin Germany D-12200 ny
Citazione:
H.J. Epple et al., "Antisecretory effect of loperamide in colon epithelial cells by inhibitionof basolateral K+ conductance", SC J GASTR, 36(7), 2001, pp. 731-737

Abstract

Background: The mechanism of the antisecrctory affect of loperamide was investigated in cultured highly differentiated colon epithelial cells (HT-29/B6). Methods: Chloride secretion was stimulated via cAMP by forskolin (FSK,10(-5) M), via Ca2+ by the muscarinic agonist carbachol (CCh, 10(-4) M), and via protein kinase C by the phorbol ester PMA (5.10(-9) M). Stimulated Cl- secretion was quantified as short circuit current (I-SC) of HT-29/B6 monolayers mounted in Ussing-type chambers, Results: Loperamide (5.10(-5) M) inhibited Ise stimulated by FSK, CCh and PMA. The antisecretory action of loperamide was unaffected by preincubation with naloxone (10(-5) M). Furthermore, loperamide strongly inhibited basolateral Rb-86 efflux. Like loperamide. the calmodulin antagonist trifluoperazine (10(-4) M) inhibited I-SC induced by FSK, CCh or PMA. The Ca2+ channel blocker verapamil (5.10(-5) M), onthe other hand, inhibited only PMA-stimulated Ise but had no effect on FSKor CCh-induced ISC Conclusions: Loperamide exerts a direct antisecretory action on chloride secretion of colon epithelial cells independently of the respective stimulatory signal transduction pathway. This antisecretory effect is not mediated by opiate receptors and reflects inhibition of basolateral K+ conductance.

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Documento generato il 06/04/20 alle ore 04:52:46