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Titolo:
Extracellular domain of YWK-II, a human sperm transmembrane protein, interacts with rat Mullerian-inhibiting substance
Autore:
Tian, XY; Sha, YS; Zhang, SM; Chen, YB; Miao, SY; Wang, LF; Koide, SS;
Indirizzi:
Peking Union Med Coll, Chinese Acad Med Sci, Natl Lab Med Mol Biol, Inst Basic Med Sci, Beijing 100005, Peoples R China Peking Union Med Coll Beijing Peoples R China 100005 05, Peoples R China WHO Collaborating Ctr Res Human Reprod, Beijing 100081, Peoples R China WHO Collaborating Ctr Res Human Reprod Beijing Peoples R China 100081 na Populat Council, Ctr Biomed Res, New York, NY 10021 USA Populat Council New York NY USA 10021 Biomed Res, New York, NY 10021 USA
Titolo Testata:
REPRODUCTION
fascicolo: 6, volume: 121, anno: 2001,
pagine: 873 - 880
SICI:
1470-1626(200106)121:6<873:EDOYAH>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL GROWTH-FACTOR; PRECURSOR PROTEIN; FALSE POSITIVES; TYROSINE KINASE; GRANULOSA-CELLS; FETAL; RECEPTOR; HORMONE; CDNA; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Wang, LF Peking Union Med Coll, Chinese Acad Med Sci, Natl Lab Med Mol Biol, Inst Basic Med Sci, 5 Dong Dan San Tiao, Beijing 100005, Peoples R ChinaPeking Union Med Coll 5 Dong Dan San Tiao Beijing Peoples R China 100005
Citazione:
X.Y. Tian et al., "Extracellular domain of YWK-II, a human sperm transmembrane protein, interacts with rat Mullerian-inhibiting substance", REPRODUCT, 121(6), 2001, pp. 873-880

Abstract

The YWK-II protein in human spermatozoa is structurally related to the beta A4-amyloid precursor protein of Alzheimer disease and has high similaritywith amyloid precusor homologues. Antibodies to the YWK-II protein agglutinate human spermatozoa and may be a potential cause of infertility. In the present study, a yeast two-hybrid system (MATCHMAKER Two-Hybrid System 2; Clontech, Pale Alto, CA) was used to screen a rat ovary cDNA library for potential ligands capable of interacting with the YWK-II component. Mullerian-inhibiting substance was found to interact with the extracellular domain ofYWK-II protein. The interaction was confirmed by a binding experiment in vitro and surface plasmon resonance assays. The recombinant Mullerian-inhibiting substance can significantly increase the viability and longevity of human spermatozoa after 5 and 22 h of incubation, presumably through binding the YWK-II component on the sperm membrane. The results of this study indicate that the YWK-II sperm membrane protein may function as a receptor for Mullerian-inhibiting substance.

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Documento generato il 29/03/20 alle ore 16:50:37