Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Determining the structure of biological macromolecules by transmission electron microscopy, single particle analysis and 3D reconstruction
Autore:
Ruprecht, J; Nield, J;
Indirizzi:
Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England Univ Cambridge Cambridge England CB2 1QW hem, Cambridge CB2 1QW, England Univ London Imperial Coll Sci Technol & Med, Dept Chem, Wolfson Labs, London SW7 2AY, England Univ London Imperial Coll Sci Technol & Med London England SW7 2AY gland
Titolo Testata:
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY
fascicolo: 3, volume: 75, anno: 2001,
pagine: 121 - 164
SICI:
0079-6107(2001)75:3<121:DTSOBM>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
ESCHERICHIA-COLI RIBOSOME; PHOTOSYSTEM-II MEMBRANES; CONTRAST TRANSFER-FUNCTION; FROZEN-HYDRATED SPECIMENS; LIGHT-HARVESTING COMPLEX; OXYGEN-EVOLVING COMPLEX; X-RAY CRYSTALLOGRAPHY; ELONGATION-FACTOR-G; 3-DIMENSIONAL RECONSTRUCTION; CRYOELECTRON MICROSCOPY;
Keywords:
transmission electron microscopy; single particle analysis; image processing; 3D reconstruction; crystallography;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
186
Recensione:
Indirizzi per estratti:
Indirizzo: Ruprecht, J Univ Cambridge, Dept Biochem, Hopkins Bldg, Cambridge CB2 1QW,England Univ Cambridge Hopkins Bldg Cambridge England CB2 1QW England
Citazione:
J. Ruprecht e J. Nield, "Determining the structure of biological macromolecules by transmission electron microscopy, single particle analysis and 3D reconstruction", PROG BIOPHY, 75(3), 2001, pp. 121-164

Abstract

Single particle analysis and 3D reconstruction of molecules imaged by transmission electron microscopy have provided a wealth of medium to low resolution structures of biological molecules and macromolecular complexes, such as the ribosome, viruses, molecular chaperones and photosystem II. In this review, the principles of these techniques are introduced in a non-mathematical way, and single particle analysis is compared to other methods used for structural studies. In particular, the recent X-ray structures of the ribosome and of ribosomal subunits allow a critical comparison of single particle analysis and X-ray crystallography. This has emphasised the rapidity with which single particle analysis can produce medium resolution structures of complexes that are difficult to crystallise. Once crystals are available. X-ray crystallography can produce structures at a much higher resolution. The great similarities now seen between the structures obtained by the twotechniques reinforce confidence in the use of single particle analysis and3D reconstruction, and show that for electron cryo-microscopy structure distortion during sample preparation and imaging has not been a significant problem, The ability to analyse conformational flexibility and the ease withwhich time-resolved studies can be performed are significant advantages for single particle analysis. Future improvements in single particle analysisand electron microscopy should increase the attainable resolution, Combining single particle analysis of macromolecular complexes and electron tomography of subcellular structures with high-resolution X-ray structures may enable us to realise the ultimate dream of structural biology a complete description of the macromolecular complexes of the cell in their different functional states, (C) 2001 Elsevier Science Ltd, All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 12:13:37