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Titolo:
In situ histochemical monitoring of ozone- and TMV-induced reactive oxygenspecies in tobacco leaves
Autore:
Rossetti, S; Bonatti, PM;
Indirizzi:
Univ Modena & Reggio Emilia, Fac Agr, I-42100 Reggio Emilia, Italy Univ Modena & Reggio Emilia Reggio Emilia Italy I-42100 io Emilia, Italy
Titolo Testata:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 5, volume: 39, anno: 2001,
pagine: 433 - 442
SICI:
0981-9428(200105)39:5<433:ISHMOO>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
OXIDATIVE BURST; PLANT DEFENSE; CELL-DEATH; INVOLVEMENT; STRESS; MECHANISMS; GENERATION; ACTIVATION; INFECTION; TISSUES;
Keywords:
hypersensitive reaction; in situ histochemistry; Nicotiana tabacum; oxidative burst; ozone; ROS; TMV;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Bonatti, PM Univ Modena & Reggio Emilia, Fac Agr, Via Kennedy 17, I-42100 Reggio Emilia, Italy Univ Modena & Reggio Emilia Via Kennedy 17 Reggio Emilia Italy I-42100
Citazione:
S. Rossetti e P.M. Bonatti, "In situ histochemical monitoring of ozone- and TMV-induced reactive oxygenspecies in tobacco leaves", PL PHYS BIO, 39(5), 2001, pp. 433-442

Abstract

The pattern and time course of reactive oxygen species (ROS) production were monitored histochemically in vivo in tobacco (Nicotiana tabacum L.) cv. Bel W3 and cv. Bel B leaves, comparing the hypersensitive (HR)-like reaction induced by the abiotic stressor ozone and the response to tobacco mosaic virus (TMV). The applied assays (nitro blue tetrazolium, NBT; 2',7'-dichlorofluorescin diacetate, DCFH-DA; 3,3'-diaminobenzidine, DAB) gave positive results, evidencing scattered ROS production in a spotted pattern. DCFH-DA and DAB predominantly evidenced localised, long-lasting hydrogen peroxide production in cells prone to die, producing very similar pictures in TMV infected leaves of both tobacco cultivars and in ozone-sensitive Eel W3. Nitro blue tetrazolium reaction pointed out that a transient oxidative burst involving superoxide anion is induced early by ozone also in the tolerant cv. Eel B, despite the low malondialdehyde level. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:03:47