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Titolo:
Determination of cDNA and genomic DNA sequences of hevamine, a chitinase from the rubber tree Hevea brasiliensis
Autore:
Bokma, E; Spiering, M; Chow, KS; Mulder, PPMFA; Subroto, T; Beintema, JJ;
Indirizzi:
Rijksuniv Groningen, Biochem Lab, NL-9747 AG Groningen, Netherlands Rijksuniv Groningen Groningen Netherlands NL-9747 AG ningen, Netherlands Rubber Res Inst Malaysia, Div Biotechnol, Kuala Lumpur, Malaysia Rubber Res Inst Malaysia Kuala Lumpur Malaysia , Kuala Lumpur, Malaysia Padjadjaran Univ, FMIPA, Lab Biokimia, Bandung, Indonesia Padjadjaran Univ Bandung Indonesia PA, Lab Biokimia, Bandung, Indonesia
Titolo Testata:
PLANT PHYSIOLOGY AND BIOCHEMISTRY
fascicolo: 5, volume: 39, anno: 2001,
pagine: 367 - 376
SICI:
0981-9428(200105)39:5<367:DOCAGD>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAX L. MERR.; LYSOZYME CHITINASE; TOBACCO; LATEX; EXPRESSION; PROTEIN; BETA-1,3-GLUCANASE; ENDOCHITINASE; INFECTION; PLANTS;
Keywords:
5 '-and 3 '-RACE; family 18 chitinase; phylogeny; Southern hybridization; PR-proteins, pathogenesis related proteins; RACE, rapid amplification of cDNA ends;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Bokma, E Rijksuniv Groningen, Biochem Lab, Nijenborgh 4, NL-9747 AG Groningen, Netherlands Rijksuniv Groningen Nijenborgh 4 Groningen Netherlands NL-9747 AG
Citazione:
E. Bokma et al., "Determination of cDNA and genomic DNA sequences of hevamine, a chitinase from the rubber tree Hevea brasiliensis", PL PHYS BIO, 39(5), 2001, pp. 367-376

Abstract

Hevamine is a chitinase from the rubber tree Hevea brasiliensis and belongs to the family 18 glycosyl hydrolases. This paper describes the cloning ofhevamine DNA and cDNA sequences. Hevamine contains a signal peptide at theN-terminus and a putative vacuolar targeting sequence at the C-terminus which are not present in the mature protein. The gene encoding hevamine contains no introns. Southern hybridization of Hevea brasiliensis DNA with a full-length hevamine cDNA probe showed that there are multiple copies of genesencoding hevamine or hevamine homologues in the Hevea brasiliensis genome. In the lutoid body (vacuolar) fraction of rubber latex only three hevaminecomponents were detected with very similar primary structures, which are probably products of one single locus on the genome. (C) 2001 Editions scientifiques et medicales Elsevier SAS.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 22:21:33