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Titolo:
Methods for studying the nematophagous fungus Verticillium chlamydosporiumin the root environment
Autore:
Hirsch, PR; Atkins, SD; Mauchline, TH; Morton, CO; Davies, KG; Kerry, BR;
Indirizzi:
IACR Rothamsted, Dept Soil Sci, Harpenden AL5 2JQ, Herts, England IACR Rothamsted Harpenden Herts England AL5 2JQ n AL5 2JQ, Herts, England IACR Rothamsted, Dept Entomol & Nematol, Harpenden AL5 2JQ, Herts, EnglandIACR Rothamsted Harpenden Herts England AL5 2JQ n AL5 2JQ, Herts, England
Titolo Testata:
PLANT AND SOIL
fascicolo: 1-2, volume: 232, anno: 2001,
pagine: 21 - 30
SICI:
0032-079X(2001)232:1-2<21:MFSTNF>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREAL CYST-NEMATODE; HETERODERA-AVENAE; SOIL; PCR; GODDARD; PLANT;
Keywords:
Verticillium chlamydosporium; biocontrol; PCR detection; beta-tubulin gene; monoclonal antibodies; immunofluorescence;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Hirsch, PR IACR Rothamsted, Dept Soil Sci, Harpenden AL5 2JQ, Herts, England IACR Rothamsted Harpenden Herts England AL5 2JQ Herts, England
Citazione:
P.R. Hirsch et al., "Methods for studying the nematophagous fungus Verticillium chlamydosporiumin the root environment", PLANT SOIL, 232(1-2), 2001, pp. 21-30

Abstract

In order to exploit fully the biocontrol potential of the nematophagous fungus Verticillium chlamydosporium, it is important to understand the ecology of the fungus in soil, and interactions with both plant and nematode hosts. Several approaches for studying the fungus in soil and the root environment are compared. These include a semi-selective medium for V. chlamydosporium, PCR primers specific for the fungal beta -tubulin gene, and monoclonalantibodies. In addition to providing a target for species-specific primers, the beta -tubulin gene is implicated in resistance to the fungicides usedin the semi-selective medium, and the genetic basis for this is investigated. Culture and PCR-based methods were used to screen for the presence of the fungus in field soils known to have been suppressive to cereal cyst nematode and that contained V. chlamydosporium populations. Monoclonal antibodies specific for either hyphae or conidia of the fungus were obtained, and their application as a tool for visualising the infection process on the root was explored.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 13:13:33