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Titolo:
THE TRANSCRIPTIONAL REPRESSOR ICER AND CAMP-INDUCED PROGRAMMED CELL-DEATH
Autore:
RUCHAUD S; SEITE P; FOULKES NS; SASSONECORSI P; LANOTTE M;
Indirizzi:
HOP ST LOUIS,CTR G HAYEM,INSERM U301,1 AVE CLAUDE VELLEFAUX F-75010 PARIS FRANCE HOP ST LOUIS,CTR G HAYEM,INSERM U301 F-75010 PARIS FRANCE INST GENET BIOL MOL & CELLULAIRE F-67404 ILLKIRCH GRAFFENS FRANCE
Titolo Testata:
Oncogene
fascicolo: 7, volume: 15, anno: 1997,
pagine: 827 - 836
SICI:
0950-9232(1997)15:7<827:TTRIAC>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
LONG-TERM DESENSITIZATION; DNA FRAGMENTATION; RETINOIC ACID; CREM GENE; RESPONSIVE-ELEMENT; CYCLIC-NUCLEOTIDES; CHOLERA-TOXIN; KINASE-I; APOPTOSIS; EXPRESSION;
Keywords:
APOPTOSIS; LEUKEMIA; CAMP-SIGNALING; TRANSCRIPTION FACTORS; CREM; ICER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
70
Recensione:
Indirizzi per estratti:
Citazione:
S. Ruchaud et al., "THE TRANSCRIPTIONAL REPRESSOR ICER AND CAMP-INDUCED PROGRAMMED CELL-DEATH", Oncogene, 15(7), 1997, pp. 827-836

Abstract

The cAMP pathway plays a central role in the response to hormonal signals for cell proliferation, differentiation and apoptosis, In IPC-81 leukaemia cells, activation of the cAMP pathway by prostaglandin E1 treatment, or other cAMP-elevating agents, induces apoptosis within 4-6 h. Inhibition of mRNA or protein synthesis during the first 2h of cAMPinduction protects cells from apoptosis, suggesting a requirement forearly gene expression. cAMP-dependent protein kinase phosphorylates aclass of nuclear factors and thereby regulates the transcription of aspecific set of genes. Here we show that CREM (cAMP Responsive Element Modulator) expression is induced rapidly upon prostaglandin E1 treatment of IPC-81 cells. The induced transcripts correspond to the early product ICER (Inducible cAMP Early Repressor), ICER expression remainselevated until the burst of cell death, Protein synthesis inhibitors which prevent cAMP-induced apoptosis also block de novo ICER synthesis, Transfected IPC-81 cell lines, constitutively expressing high level of ICER are resistant to cAMP-induced cell death, In these transfectedcells, cAMP fails to upregulate the ICER transcripts demonstrating that ICER exerts strongly its repressor function on CRE-containing genes. That an early expression of ICER blocks apoptosis, suggests that gene repression by endogenous ICER in IPC-81 is unsufficient or occurs too late to protect cells against death. ICER transfected cells rescued from cAMP-induced apoptosis are growth arrested, It shows for the first time that CREM activation directly participates to the decision of the cell to die. ICER, by sequentially repressing distinct sets of CRE-containing genes could modulate cell fate.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 11:09:33