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Titolo:
The neuromodulatory effects of VIP/PACAP on PC-12 cells are associated with their N-terminal structures
Autore:
Onoue, S; Waki, Y; Nagano, Y; Satoh, S; Kashimoto, K;
Indirizzi:
Itoham Foods Inc, Cent Res Inst, Moriya, Ibaraki 3020104, Japan Itoham Foods Inc Moriya Ibaraki Japan 3020104 iya, Ibaraki 3020104, Japan Toho Univ, Sch Pharmaceut Sci, Chiba 2748510, Japan Toho Univ Chiba Japan 2748510 , Sch Pharmaceut Sci, Chiba 2748510, Japan
Titolo Testata:
PEPTIDES
fascicolo: 6, volume: 22, anno: 2001,
pagine: 867 - 872
SICI:
0196-9781(200106)22:6<867:TNEOVO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLASE-ACTIVATING POLYPEPTIDE; NERVE GROWTH-FACTOR; VASOACTIVE-INTESTINAL-PEPTIDE; STIMULATES ADENYLATE-CYCLASE; NUCLEAR-MAGNETIC-RESONANCE; PC12 CELLS; MOLECULAR-CLONING; NEURITE OUTGROWTH; PACAP RECEPTOR; PITUITARY;
Keywords:
VIP; PACAP; PC-12 cells; neurite outgrowth; neuropeptides; molecular dynamics; structure-activity relationships;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Onoue, S Itoham Foods Inc, Cent Res Inst, Moriya, Ibaraki 3020104, Japan Itoham Foods Inc Moriya Ibaraki Japan 3020104 aki 3020104, Japan
Citazione:
S. Onoue et al., "The neuromodulatory effects of VIP/PACAP on PC-12 cells are associated with their N-terminal structures", PEPTIDES, 22(6), 2001, pp. 867-872

Abstract

The current study explored whether the differences in biological activities in PC-12 cells between vasoactive intestinal polypeptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) are attributable to the sequence difference in their N-terminal portions and are correlated with the solution structures of the peptides. In the neurite outgrowth assay, N-terminal modification of VIP to PACAP-like sequences altered its effect, the activity was confirmed even at a low concentration (10(-10) M). On the contrary, N-terminal modification of PACAP 27 to VIP-like sequences reduced its activity. These relationships were also confirmed for the inhibitory effects: of the peptide analogues on PC-12 cells growth at 10(-7) M. The present results combined with our previously reported data, including binding assay, support that the N-termini of VIP/PACAP plays an important role in their activities. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 07:16:03