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Titolo:
Urine-based enzyme-linked immunosorbent assay for the detection of Helicobacter pylori infection in children
Autore:
Kato, S; Tachikawa, T; Ozawa, K; Konno, M; Okuda, M; Fujisawa, T; Nakazato, Y; Tajiri, H; Iinuma, K;
Indirizzi:
Tohoku Univ, Sch Med, Dept Pediat, Aoba Ku, Sendai, Miyagi 9808574, Japan Tohoku Univ Sendai Miyagi Japan 9808574 Ku, Sendai, Miyagi 9808574, Japan Otsuka Pharmaceut Co Ltd, Dept Diagnost Reagents, Tokushima 77101, Japan Otsuka Pharmaceut Co Ltd Tokushima Japan 77101 s, Tokushima 77101, Japan Sapporo Kousei Gen Hosp, Sapporo, Hokkaido, Japan Sapporo Kousei Gen HospSapporo Hokkaido Japan Sapporo, Hokkaido, Japan Wakayama Rosai Hosp, Wakayama, Japan Wakayama Rosai Hosp Wakayama JapanWakayama Rosai Hosp, Wakayama, Japan Kurume Univ, Sch Med, Kurume, Fukuoka 830, Japan Kurume Univ Kurume Fukuoka Japan 830 Sch Med, Kurume, Fukuoka 830, Japan Social Insurance Ohmiya Gen Hosp, Ohmiya, Japan Social Insurance Ohmiya Gen Hosp Ohmiya Japan a Gen Hosp, Ohmiya, Japan Osaka Univ, Sch Med, Suita, Osaka 565, Japan Osaka Univ Suita Osaka Japan565 a Univ, Sch Med, Suita, Osaka 565, Japan
Titolo Testata:
PEDIATRICS
fascicolo: 6, volume: 107, anno: 2001,
pagine: NIL_6 - NIL_10
SICI:
0031-4005(200106)107:6<NIL_6:UEIAFT>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
DYSPEPTIC PATIENTS; IMMUNOGLOBULIN-G; HEART-DISEASE; ANTIBODY; IMMUNOASSAY; ERADICATION; ASSOCIATION; GASTRITIS; DIAGNOSIS; ANEMIA;
Keywords:
enzyme-linked immunosorbent assay; Helicobacter pylori; immunoglobulin G; urine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Kato, S Tohoku Univ, Sch Med, Dept Pediat, Aoba Ku, 1-1 Seiryo Machi, Sendai, Miyagi 9808574, Japan Tohoku Univ 1-1 Seiryo Machi Sendai Miyagi Japan 9808574 74, Japan
Citazione:
S. Kato et al., "Urine-based enzyme-linked immunosorbent assay for the detection of Helicobacter pylori infection in children", PEDIATRICS, 107(6), 2001, pp. NIL_6-NIL_10

Abstract

Objective. Serology and C-13-urea breath test have been widely used as noninvasive tests to detect Helicobacter pylori infection. However, easier collection of samples and lower costs are desirable for diagnosis of the individual patient or for use in epidemiologic studies. Our aim was to study thediagnostic accuracy of a recently developed urine-based enzyme-linked immunosorbent assay (ELISA) kit for the detection of H pylori-specific immunoglobulin G (IgG) antibodies in children. Study Design. Specimens of serum and randomly voided urine were collected from 816 children (0-15 years old) and were analyzed using 2 serum-based ELISA kits and a urine-based ELISA kit, respectively. Based on results of serology, the sensitivity, specificity, and accuracy of the urine-based ELISA kit were evaluated. With regard to false-positive and false-negative results, urinary IgG concentrations and IgG/creatinine levels were studied. Results. Both serum-based ELISAs were positive in 41 children and were negative in 666, who were enrolled in this study. The remaining 109 children were excluded because of disagreement between the results of the 2 serum-based ELISAs, including indeterminate values. Overall sensitivity, specificity, and accuracy of urine-based ELISA test compared with serology were 85.4%,95.5%, and 94.9%, respectively. On positivity rates, the urine-based ELISAwas closely coincident with the serum-based ELISA in each age group. Therewas no correlation between antibody levels detected by urine-based ELISA and each serum-based ELISA. Urinary IgG concentrations and IgG/creatinine levels were significantly higher in false-positives and were lower in false-negatives than in true-positives plus true-negatives for serology. Most of those with false-positive results had trace to moderate proteinuria. Conclusions. The urine-based ELISA is an alternative to serum-based ELISA for diagnosis of H pylori infection in children and should be suitable for large-scale epidemiologic studies concerning the organism. In children withproteinuria, results of the test should be interpreted with caution. It ispossible that the urine-based ELISA method would be applicable to diagnosis of other infectious diseases.

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Documento generato il 01/10/20 alle ore 16:23:21