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Titolo:
Structural and functional properties of hemocyanin from Cyanagraea praedator, a deep-sea hydrothermal vent crab
Autore:
Chausson, F; Bridges, CR; Sarradin, PM; Green, BN; Riso, R; Caprais, JC; Lallier, FH;
Indirizzi:
UPMC, Observ Oceanol Roscoff, CNRS, UPR 9042, Roscoff, France UPMC Roscoff France rv Oceanol Roscoff, CNRS, UPR 9042, Roscoff, France IFREMER, DRO, EP, Plouzane, France IFREMER Plouzane FranceIFREMER, DRO, EP, Plouzane, France Univ Dusseldorf, Inst Zoophysiol, D-4000 Dusseldorf, Germany Univ Dusseldorf Dusseldorf Germany D-4000 ol, D-4000 Dusseldorf, Germany UBO, Lab Chim Marine, Plouzane, France UBO Plouzane FranceUBO, Lab Chim Marine, Plouzane, France IUEM, CNRS, UPR 9042, Plouzane, France IUEM Plouzane FranceIUEM, CNRS, UPR 9042, Plouzane, France
Titolo Testata:
PROTEINS-STRUCTURE FUNCTION AND GENETICS
fascicolo: 4, volume: 45, anno: 2001,
pagine: 351 - 359
SICI:
0887-3585(200112)45:4<351:SAFPOH>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
WORM RIFTIA-PACHYPTILA; OXYGEN-AFFINITY; CARCINUS-MAENAS; SHORE CRAB; CALLINECTES-SAPIDUS; L-LACTATE; BINDING; CRUSTACEANS; TRANSPORT; PROTEINS;
Keywords:
respiratory pigments; crustaceans; hypoxia; quaternary structure; ESI-MS; oxygen affinity; cooperativity; Bohr effect; temperature;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Chausson, F CNRS, Biol Stn, Equipe Ecophysiol, BP 74, F-29682 Roscoff, France CNRS BP 74 Roscoff France F-29682 74, F-29682 Roscoff, France
Citazione:
F. Chausson et al., "Structural and functional properties of hemocyanin from Cyanagraea praedator, a deep-sea hydrothermal vent crab", PROTEINS, 45(4), 2001, pp. 351-359

Abstract

Cyanagraea praedator (Crustacea: Decapoda: Brachyura) is an endemic species of the East Pacific Rise hydrothermal vents, living in the upper part of black smoker chimneys. Because we were seeking species that have made respiratory adaptations to the hydrothermal environment, we looked at Cyanograeahemocyanin (Hc) and determined its quaternary structure and the oxygen-binding properties in relation to temperature, pH, and lactate. C. praedator Hc is composed of dodecamers and hexamers, with dodecamers formed by the perpendicular association of two hexamers. The composition of these polymers was determined by electrophoresis and, for the first time, by electrospray mass spectrometry. Dodecamers and hexamers are composed of six subunits common to the two forms, with molecular mass ranging from 75,008 Da to 75,534 Da. In addition, we found two dodecamer-specific subunits, at 75,419 Da and 75,629 Da. The native hemocyanin possesses a high oxygen affinity (P-50 varies between 4 and 10 Torr at pH 7.5, 15 degreesC) and a large Bohr coefficient (A log P-50/Delta pH similar to - 1.8). Oxygen affinity is not affectedby lactate or, surprisingly, temperature between 5 degreesC and 35 degreesC (DeltaH = 1.16 kJ/mol(1) 5-35 degreesC). Dialysis of native hemolymph elicited a significant increase in Hc-O-2 affinity (DeltaP(50) = 2.5 Torr at pH 7.5), an effect opposite the usual trend observed for crustacean hemocyanins. In this article these functional properties are interpreted in relation to characteristics of the environment. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 08:38:46