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Titolo:
Simultaneous determination of multiple components in lactic acid fermentation using FT-MIR, NIR, and FT-Raman spectroscopic techniques
Autore:
Sivakesava, S; Irudayaraj, J; Ali, D;
Indirizzi:
Penn State Univ, Dept Agr & Biol Engn, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 n, University Pk, PA 16802 USA
Titolo Testata:
PROCESS BIOCHEMISTRY
fascicolo: 4, volume: 37, anno: 2001,
pagine: 371 - 378
SICI:
1359-5113(200112)37:4<371:SDOMCI>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEAR-INFRARED SPECTROSCOPY; ESCHERICHIA-COLI FERMENTATION; MIDINFRARED SPECTROSCOPY; CALIBRATION; IDENTIFICATION; BACTERIA; GLUCOSE; AMMONIA; LACTATE;
Keywords:
Fourier transform mid infrared spectroscopy; near infrared spectroscopy; Fourier transform Raman spectroscopy; fermentation; lactic acid; Lactobacillus casei;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
16
Recensione:
Indirizzi per estratti:
Indirizzo: Irudayaraj, J Penn State Univ, Dept Agr & Biol Engn, 249 Agr Engn Bldg, University Pk, PA 16802 USA Penn State Univ 249 Agr Engn Bldg University Pk PA USA 16802
Citazione:
S. Sivakesava et al., "Simultaneous determination of multiple components in lactic acid fermentation using FT-MIR, NIR, and FT-Raman spectroscopic techniques", PROCESS BIO, 37(4), 2001, pp. 371-378

Abstract

Bioprocess optimization is often limited by its inability to measure biomass, nutrients and product concentrations in a time frame that allows process adjustments. Infrared spectroscopic techniques can potentially be used tomeasure each of these components within about 3 min of sampling, of an unprocessed whole broth sample. In the present study, the use of Fourier transform mid infrared (FT-MIR), near infrared red (NIR), and Fourier transform (FT)-Raman spectroscopic techniques for at-line (rapid off-line) monitoringof biomass, glucose, and lactic acid in a lactic acid fermentation by Lactobacillus casei was investigated. Suitable spectral wavenumber regions wereselected for principal least square (PLS) regression and principal component regression (PCR) and calibration models were developed. The standard error of calibration for the PLS-1st derivative calibration models for glucose, lactic acid, and optical cell density were 2.5, 0.7 g/l, and 0.23, respectively. Prediction errors were high for NIR and FT-Raman methods. Results indicated that FT-MIR spectroscopy could be used for rapid detection of glucose, lactic acid, and optical cell density during lactic acid fermentation. (C) 2001 Elsevier Science Ltd. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/04/20 alle ore 01:56:10