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Titolo:
Levan production by use of the recombinant levansucrase immobilized on titanium-activated magnetite
Autore:
Jang, KH; Song, KB; Park, BS; Kim, CH; Chung, BH; Choue, RW; Lee, KS; Lee, C; Chun, UH; Rhee, SK;
Indirizzi:
Korea Res Inst Biosci & Biotechnol, Biotechnol Res Div, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol Taejon South Korea 305600 South Korea Kyung Hee Univ, Coll Hotel & Tourism, Grad Sch EW Med Sci, Dept Med Nutr, Seoul 130701, South Korea Kyung Hee Univ Seoul South Korea 130701 Nutr, Seoul 130701, South Korea Kyung Hee Univ, Coll Hotel & Tourism, Dept Culinary Sci, Seoul 130701, South Korea Kyung Hee Univ Seoul South Korea 130701 y Sci, Seoul 130701, South Korea Hanseo Univ, Dept Food & Biotechnol, Seosan 356820, Chungnam, South Korea Hanseo Univ Seosan Chungnam South Korea 356820 820, Chungnam, South Korea Kyung Hee Univ, Inst Life Sci, Dept Food Sci & Technol, Suwon 449701, South Korea Kyung Hee Univ Suwon South Korea 449701 chnol, Suwon 449701, South Korea
Titolo Testata:
PROCESS BIOCHEMISTRY
fascicolo: 4, volume: 37, anno: 2001,
pagine: 339 - 343
SICI:
1359-5113(200112)37:4<339:LPBUOT>2.0.ZU;2-4
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZYMOMONAS-MOBILIS; EXTRACELLULAR LEVANSUCRASE; PURIFICATION; POLYMERASE;
Keywords:
immobilization; levan; levansucrase; titanium-activated magnetite; Zymomonas mobilis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Rhee, SK Korea Res Inst Biosci & Biotechnol, Biotechnol Res Div, POB 115, Taejon 305600, South Korea Korea Res Inst Biosci & Biotechnol POB 115 Taejon South Korea 305600
Citazione:
K.H. Jang et al., "Levan production by use of the recombinant levansucrase immobilized on titanium-activated magnetite", PROCESS BIO, 37(4), 2001, pp. 339-343

Abstract

Levansucrase from Zymomonas mobilis expressed in Escherichia coli was immobilized onto the surface of magnetite. Optimum conditions for the immobilization were, pH 4.0; immobilization reaction time of 30 min and 8 U of enzyme per gram of matrix. Immobilization yield was 75% under optimized conditions. The maximum production yield of levan by the immobilized levansucrase was about 42% on a fructose released basis with sucrose as substrate at 100 g/l. Thermal stability of the levansucrase increased by immobilization procedure. Furthermore, immobilized enzyme showed the less polymerizing activity in levan formation, producing the low-molecular weight (MW) levan. The molecular weight of levan can be controlled by using the immobilized levansucrase in high yield. Immobilized levansucrase retained 61% of the original activity after five repeated uses. (C) 2001 Elsevier Science Ltd. All rightsreserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 02:21:33