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Titolo:
Ryanodine-, IP3- and NAADP-dependent calcium stores control acetylcholine release
Autore:
Chameau, P; Van de Vrede, Y; Fossier, P; Baux, G;
Indirizzi:
CNRS, Neurobiol Cellulaire & Mol Lab, F-91198 Gif Sur Yvette, France CNRSGif Sur Yvette France F-91198 l Lab, F-91198 Gif Sur Yvette, France
Titolo Testata:
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
fascicolo: 2, volume: 443, anno: 2001,
pagine: 289 - 296
SICI:
0031-6768(200111)443:2<289:RIANCS>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYCLIC ADP-RIBOSE; ADENINE-DINUCLEOTIDE PHOSPHATE; MUSCLE SARCOPLASMIC-RETICULUM; POST-SPIKE HYPERPOLARIZATION; CA2+-INDUCED CA2+ RELEASE; VAGAL AFFERENT NEURONS; NEUROTRANSMITTER RELEASE; TRANSMITTER RELEASE; INOSITOL TRISPHOSPHATE; SYNAPTIC VESICLES;
Keywords:
acetylcholine release; cADP-ribose; Ca2+ stores; IP3; NAADP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Baux, G CNRS, Neurobiol Cellulaire & Mol Lab, F-91198 Gif Sur Yvette, France CNRS Gif Sur Yvette France F-91198 -91198 Gif Sur Yvette, France
Citazione:
P. Chameau et al., "Ryanodine-, IP3- and NAADP-dependent calcium stores control acetylcholine release", PFLUG ARCH, 443(2), 2001, pp. 289-296

Abstract

Injections of inositol trisphosphate (IP3) or nicotinamide adenine dinucleotide phosphate (NAADP) into the presynaptic neurone of an identified cholinergic synapse in the buccal ganglion of Aplysia californica increased the amplitude of the inhibitory postsynaptic current evoked by a presynaptic action potential. This suggests that Ca2+ release from various Ca2+ stores can modulate acetylcholine (ACh) release. Specific blockade of the calcium-induced calcium release (CICR) mechanism with ryanodine, or of IP3-induced calcium release with heparin, abolished the effects Of IP3, but not the effects of NAADP, suggesting the presence of an intracellular Ca2+ pool independent of those containing ryanodine receptors (RyR) or IP3 receptors. To reinforce electrophysiological observations, intracellular [Ca2+], changes weremeasured using the fluorescent dye rhod-2. Injections of cyclic ADP-ribose(an activator of RyR), IP3 or NAADP into the presynaptic neurone induced transient increases in the free intracellular Ca2+ concentration. RyR- and IP3-induced increases were prevented by application of respective selective antagonists but not NAADP-induced increases. Our results show that RyR-dependent, IP3-dependent, and NAADP-dependent Ca2+ stores are present in the same presynaptic terminal but are differently involved in the regulation of the presynaptic Ca2+ concentration that triggers transmitter release.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 21:45:43