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Titolo:
Characterization of stage progression in chronic myeloid leukemia by DNA microarray with purified hematopoietic stem cells
Autore:
Ohmine, K; Ota, J; Ueda, M; Ueno, S; Yoshida, K; Yamashita, Y; Kirito, K; Imagawa, S; Nakamura, Y; Saito, K; Akutsu, M; Mitani, K; Kano, Y; Komatsu, N; Ozawa, K; Mano, H;
Indirizzi:
Jichi Med Sch, Div Funct Genom, Tochigi 3290498, Japan Jichi Med Sch Tochigi Japan 3290498 Funct Genom, Tochigi 3290498, Japan Jichi Med Sch, Div Hematol, Tochigi 3290498, Japan Jichi Med Sch TochigiJapan 3290498 Div Hematol, Tochigi 3290498, Japan Jichi Med Sch, Div Cardiol, Tochigi 3290498, Japan Jichi Med Sch TochigiJapan 3290498 Div Cardiol, Tochigi 3290498, Japan Univ Tsukuba, Sch Med, Dept Hematol, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan Dokkyo Univ, Sch Med, Dept Hematol, Mibu, Tochigi 3210293, Japan Dokkyo Univ Mibu Tochigi Japan 3210293 atol, Mibu, Tochigi 3210293, Japan Tochigi Canc Ctr, Utsunomiya, Tochigi 3200834, Japan Tochigi Canc Ctr Utsunomiya Tochigi Japan 3200834 Tochigi 3200834, Japan
Titolo Testata:
ONCOGENE
fascicolo: 57, volume: 20, anno: 2001,
pagine: 8249 - 8257
SICI:
0950-9232(200112)20:57<8249:COSPIC>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
EXPRESSION; GENE; CDNA; INTERFERON; PROTEINS; CLONING; TUMOR;
Keywords:
chronic myeloid leukemia; DNA microarray; AC133; PIASy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Mano, H Jichi Med Sch, Div Funct Genom, 3311-1 Yakushiji, Tochigi 3290498,Japan Jichi Med Sch 3311-1 Yakushiji Tochigi Japan 3290498 90498, Japan
Citazione:
K. Ohmine et al., "Characterization of stage progression in chronic myeloid leukemia by DNA microarray with purified hematopoietic stem cells", ONCOGENE, 20(57), 2001, pp. 8249-8257

Abstract

Chronic myeloid leukemia (CML) is characterized by the clonal expansion ofhematopoietic stem cells (HSCs). Without effective treatment, individuals in the indolent, chronic phase (CP) of CML undergo blast crisis (BC), the prognosis for which is poor. It is therefore important to clarify the mechanism underlying stage progression in CML. DNA microarray is a versatile toolfor such a purpose. However, simple comparison of bone marrow mononuclear cells from individuals at different disease stages is likely to result in the identification of pseudo-positive genes whose change in expression only reflects the different proportions of leukemic blasts in bone marrow. We have therefore compared with DNA microarray the expression profiles of 3456 genes in the purified HSC-like fractions that had been isolated from 13 CML patients and healthy volunteers. Interestingly, expression of the gene for PIASy, a potential inhibitor of STAT (signal transducer and activator of transcription) proteins, was down-regulated in association with stage progression in CML. Furthermore, forced expression of PIASy has induced apoptosis in a CML cell line. These data suggest that microarray analysis with background-matched samples is an efficient approach to identify molecular events underlying the stage progression in CML.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 00:14:02