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Titolo:
Interleukin-1 beta-induced promatrilysin expression is mediated by NF kappa B-regulated synthesis of interleukin-6 in the prostate carcinoma cell line, LNCaP
Autore:
Maliner-Stratton, MS; Klein, RD; Udayakumar, TS; Nagle, RB; Bowden, GT;
Indirizzi:
Univ Arizona, Hlth Sci Ctr, Dept Radiat Oncol, Tucson, AZ 85724 USA Univ Arizona Tucson AZ USA 85724 Dept Radiat Oncol, Tucson, AZ 85724 USA Univ Texas, MD Anderson Canc Ctr, Dept Carcinogenesis, Smithville, TX 78957 USA Univ Texas Smithville TX USA 78957 rcinogenesis, Smithville, TX 78957 USA
Titolo Testata:
NEOPLASIA
fascicolo: 6, volume: 3, anno: 2001,
pagine: 509 - 520
SICI:
1522-8002(200111/12)3:6<509:IBPEIM>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATRIX METALLOPROTEINASE MATRILYSIN; NON-HODGKINS-LYMPHOMAS; HUMAN-MELANOMA CELLS; TISSUE INHIBITOR; GROWTH-FACTOR; CANCER CELLS; IN-VITRO; OSTEOCLAST DIFFERENTIATION; TRANSCRIPTION FACTOR; AUTOCRINE GROWTH;
Keywords:
matrilysin; prostate; matrix metalloproteinase; interleukin-6; interleukin-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Bowden, GT Univ Arizona, Arizona Canc Ctr, Dept Radiat Oncol, Tucson, AZ 85724 USA Univ Arizona Tucson AZ USA 85724 t Oncol, Tucson, AZ 85724 USA
Citazione:
M.S. Maliner-Stratton et al., "Interleukin-1 beta-induced promatrilysin expression is mediated by NF kappa B-regulated synthesis of interleukin-6 in the prostate carcinoma cell line, LNCaP", NEOPLASIA, 3(6), 2001, pp. 509-520

Abstract

Previously, our laboratory showed that interleukin-1 beta (IL-1 beta) secreted by lipopolysaccharide-activated monocytes induces promatrilysin expression in the prostate carcinoma cell line, LNCaP. We now demonstrate that IL-1 beta -induced promatrilysin expression is mediated by an indirect mechanism that requires nuclear factor Kappa B (NF kappaB)- dependent synthesis of IL-6. Inhibition of protein synthesis with cyclohexamide blocked IL-1 beta -mediated induction of matrilysin mRNA suggesting that synthesis of one or more additional factors is required for IL-1 beta -induced promatrilysin protein expression. Blockage of NF kappaB transactivation activity abrogated IL-1 beta -induced promatrilysin expression to baseline levels suggestingthat NF kappaB transactivation activity is necessary. Inhibition of IL-6 activity attenuated IL-1 beta -induced promatrilysin, but not NF kappaB transactivation activity indicating that IL-6 acts downstream of NF kappaB in potentiation of IL-1 beta -mediated promatrilysin expression. Inhibition of protein synthesis with cyclohexamide did not alter IL-6-induced induction of matrilysin mRNA indicating that, contrary to the mechanism by which IL-1 beta regulates promatrilysin expression, IL-6-mediated matrilysin mRNA expression does not require new protein synthesis. Transient transfection with dominant negative STAT3 inhibited IL-1 beta- and IL-6-induced promatrilysin. These data provide evidence that NF kappaB-mediated IL-6 synthesis is required for IL-1 beta -induced promatrilysin expression, and IL-6 signaling through STAT3 plays a role in IL-1 beta -induced promatrilysin expression.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 12:18:38