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Titolo:
The beta(2) subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney
Autore:
Behrends, S; Budaeus, L; Kempfert, J; Scholz, H; Starbatty, J; Vehse, K;
Indirizzi:
Univ Hamburg, Inst Expt, D-20251 Hamburg, Germany Univ Hamburg Hamburg Germany D-20251 Inst Expt, D-20251 Hamburg, Germany Univ Hamburg, Klin Pharmakol, D-20251 Hamburg, Germany Univ Hamburg Hamburg Germany D-20251 Pharmakol, D-20251 Hamburg, Germany
Titolo Testata:
NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY
fascicolo: 6, volume: 364, anno: 2001,
pagine: 573 - 576
SICI:
0028-1298(200112)364:6<573:TBSONO>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
THICK ASCENDING LIMB; NA-K-ATPASE; CONCENTRATING CAPACITY; EXPRESSION; CLONING;
Keywords:
kidney; development; soluble guanylyl cyclase; nitric oxide;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Behrends, S Univ Hamburg, Inst Expt, Martinistr 52, D-20251 Hamburg, Germany Univ Hamburg Martinistr 52 Hamburg Germany D-20251 g, Germany
Citazione:
S. Behrends et al., "The beta(2) subunit of nitric oxide-sensitive guanylyl cyclase is developmentally regulated in rat kidney", N-S ARCH PH, 364(6), 2001, pp. 573-576

Abstract

We have recently shown that nitric oxide activates the beta (2) subunit ofsoluble guanylyl cyclase. In the present study, we show developmental regulation of this subunit. Analysis of mRNA expression by RT-PCR and RNase protection analysis in kidneys revealed no expression of the beta (2) subunit in neonatal and strong expression in adult rats. A reciprocal regulation with much lower expression levels was observed in rat lung. Further examination of kidneys from 3, 6, 16, 22, 25, 31 and 36-day-old rats showed that significant expression appears between postnatal day 16 and 22. Isolation of the rat beta (2) promoter by genome walking and cloning into a reporter genevector showed promoter activity for the sense but not the antisense construct providing an in vitro assay for further analysis of the developmental beta (2) subunit regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 14:26:27