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Titolo:
Genetic mapping with SNP markers in Drosophila
Autore:
Berger, J; Suzuki, T; Senti, KA; Stubbs, J; Schaffner, G; Dickson, BJ;
Indirizzi:
Res Inst Mol Pathol, A-1030 Vienna, Austria Res Inst Mol Pathol Vienna Austria A-1030 Pathol, A-1030 Vienna, Austria
Titolo Testata:
NATURE GENETICS
fascicolo: 4, volume: 29, anno: 2001,
pagine: 475 - 481
SICI:
1061-4036(200112)29:4<475:GMWSMI>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENOME; MELANOGASTER; MOSAICS; DISCOVERY; SEQUENCE; KINASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
23
Recensione:
Indirizzi per estratti:
Indirizzo: Dickson, BJ Res Inst Mol Pathol, Dr Bohr Gasse 7, A-1030 Vienna, Austria Res Inst Mol Pathol Dr Bohr Gasse 7 Vienna Austria A-1030 ria
Citazione:
J. Berger et al., "Genetic mapping with SNP markers in Drosophila", NAT GENET, 29(4), 2001, pp. 475-481

Abstract

Map-based positional cloning of Drosophila melanogaster genes is hampered by both the time-consuming, error-prone nature of traditional methods for genetic mapping and the difficulties in aligning the genetic and cytologicalmaps with the genome sequence. The identification of sequence polymorphisms in the Drosophila genome will make it possible to map mutations directly to the genome sequence with high accuracy and resolution. Here we report the identification of 7,223 single-nucleotide polymorphisms (SNPs) and 1,392 insertions/deletions (InDels) in common laboratory strains of Drosophila. These sequence polymorphisms define a map of 787 autosomal marker loci with a resolution of 114 kb. We have established PCR product-length polymorphism(PLP) or restriction fragment-length polymorphism (RFLP) assays for 215 ofthese markers. We demonstrate the use of this map by delimiting two mutations to intervals of 169 kb and 307 kb, respectively. Using a local high-density SNP map, we also mapped a third mutation to a resolution of approximately 2 kb, sufficient to localize the mutation within a single gene. These methods should accelerate the rate of positional cloning in Drosophila.

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Documento generato il 20/01/20 alle ore 22:09:29