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Titolo:
Differential contribution of cytoplasmic Ca2+ and Ca2+ influx to gamete fusion and egg activation in maize
Autore:
Antoine, AF; Faure, JE; Dumas, C; Feijo, JA;
Indirizzi:
Univ Sci & Technol Lille, Dev Biol Lab, F-59655 Villeneuve Dascq, France Univ Sci & Technol Lille Villeneuve Dascq France F-59655 e Dascq, France Univ Lisbon, Fac Ciencias, Ctr Biotecnol Vegetal, PT-1749016 Lisbon, Portugal Univ Lisbon Lisbon Portugal PT-1749016 etal, PT-1749016 Lisbon, Portugal Gulbenkian Inst Sci, PT-2780156 Oeiras, Portugal Gulbenkian Inst Sci Oeiras Portugal PT-2780156 -2780156 Oeiras, Portugal Univ Lyon 1, Ecole Normale Super Lyon, CNRS, INRA,UMR 5667,Lab Reprod & Dev Plantes, F-69364 Lyon 07, France Univ Lyon 1 Lyon France 07 Reprod & Dev Plantes, F-69364 Lyon 07, France
Titolo Testata:
NATURE CELL BIOLOGY
fascicolo: 12, volume: 3, anno: 2001,
pagine: 1120 - 1123
SICI:
1465-7392(200112)3:12<1120:DCOCCA>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEA-URCHIN EGGS; IN-VITRO FERTILIZATION; FLOWERING PLANTS; VOLTAGE CLAMP; SPERM ENTRY; CALCIUM; WAVE; ZYGOTE; EVENTS; CELLS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Antoine, AF Univ Sci & Technol Lille, Dev Biol Lab, SN3, F-59655 Villeneuve Dascq, France Univ Sci & Technol Lille SN3 Villeneuve Dascq France F-59655
Citazione:
A.F. Antoine et al., "Differential contribution of cytoplasmic Ca2+ and Ca2+ influx to gamete fusion and egg activation in maize", NAT CELL BI, 3(12), 2001, pp. 1120-1123

Abstract

In multicellular organisms, gamete fusion triggers a set of events, collectively known as egg activation, that leads to the development of a new individual. Every species that has been studied shows at least one rise in cytoplasmic Ca2+ concentration ([Ca2+]cyt) after gamete fusion(1) which is believed to be involved in activation. Yet the source and regulation of this Ca2+ signal and the way it is transduced inside the zygote are controversial(1). In higher plants, in vitro fertilization (IVF)(2,3) has enabled the description of a rise in [Ca2+] (ref. 4) that is sufficient for activation(5),and of a Ca2+ influx that spreads as a wavefront from the fusion site(5) The relationship between these two responses is unknown. Using a new combination of methods that simultaneously monitor the extracellular flux with a Ca2+-vibrating probe, and [Ca2+](Cyt) by widefield imaging, we directly determined that the Ca2+ influx precedes the [Ca2+](Cyt) elevation by 40-120 s. In addition, results from experiments using the Ca2+-channel inhibitor gadolinium (Gd3+) suggest that the Ca2+ influx may be necessary for sperm incorporation. We also present evidence for a putative sperm-dependent Gd3+-insensitive localized Ca2+ influx confined to the fusion point.

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Documento generato il 09/07/20 alle ore 18:10:16