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Titolo:
Production of calves from G1 fibroblasts
Autore:
Kasinathan, P; Knott, JG; Wang, ZD; Jerry, DJ; Robl, JM;
Indirizzi:
Hematech LLC, Worcester, MA 01605 USA Hematech LLC Worcester MA USA 01605Hematech LLC, Worcester, MA 01605 USA Univ Massachusetts, Dept Vet & Anim Sci, Amherst, MA 01003 USA Univ Massachusetts Amherst MA USA 01003 & Anim Sci, Amherst, MA 01003 USA Hematech LLC, Manhattan, KS 66502 USA Hematech LLC Manhattan KS USA 66502Hematech LLC, Manhattan, KS 66502 USA
Titolo Testata:
NATURE BIOTECHNOLOGY
fascicolo: 12, volume: 19, anno: 2001,
pagine: 1176 - 1178
SICI:
1087-0156(200112)19:12<1176:POCFGF>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
PARTHENOGENETIC DEVELOPMENT; NUCLEAR TRANSFER; MAMMALIAN-CELLS; BOVINE OOCYTES; FETAL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Robl, JM Hematech LLC, 4 Biotech Pk,377 Plantat Dr, Worcester, MA 01605 USA Hematech LLC 4 Biotech Pk,377 Plantat Dr Worcester MA USA 01605 A
Citazione:
P. Kasinathan et al., "Production of calves from G1 fibroblasts", NAT BIOTECH, 19(12), 2001, pp. 1176-1178

Abstract

Since the landmark study of Wilmut et al.(1) describing the birth of a cloned lamb derived from a somatic cell nucleus, there has been debate about the donor nucleus cell cycle stage required for somatic cell nuclear transfer (NT). Wilmut et al.(1) suggested that induction of quiescence by serum starvation was critical in allowing donor somatic cells to support development of cloned embryos. In a subsequent report, Cibelli et al.(2) proposed that G0 was unnecessary and that calves could be produced from actively dividing fibroblasts. Neither study conclusively documented the importance of donor cell cycle stage for development to term. Other laboratories have had success with NT in several species(1-7), and most have used a serum starvation treatment. Here we evaluate methods for producing G0 and G1 cell populations and compare development following NT. High confluence was more effective than serum starvation for arresting cells in G0. Pure G1 cell populationscould be obtained using a "shake-off" procedure. No differences in in vitro development were observed between cells derived from the high-confluence treatment and from the "shake-off" treatment. However, when embryos from each treatment were transferred to 50 recipients, five calves were obtained from embryos derived from "shake-off" cells, whereas no embryos from confluent cells survived beyond 180 days of gestation. These results indicate thatdonor cell cycle stage is important for NT, particularly during late fetaldevelopment, and that actively dividing G1 cells support higher development rates than cells in G0.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 07:03:12