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Titolo:
Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells
Autore:
Bulte, JWM; Douglas, T; Witwer, B; Zhang, SC; Strable, E; Lewis, BK; Zywicke, H; Miller, B; van Gelderen, P; Moskowitz, BM; Duncan, ID; Frank, JA;
Indirizzi:
NIH, Lab Diagnost Radiol Res CC, Bethesda, MD 20892 USA NIH Bethesda MD USA 20892 Diagnost Radiol Res CC, Bethesda, MD 20892 USA Temple Univ, Dept Chem, Philadelphia, PA 19122 USA Temple Univ Philadelphia PA USA 19122 pt Chem, Philadelphia, PA 19122 USA Univ Wisconsin, Sch Vet Med, Dept Med Sci, Madison, WI 53705 USA Univ Wisconsin Madison WI USA 53705 , Dept Med Sci, Madison, WI 53705 USA NINCDS, Adv Magnet Resonance Imaging, NIH, Bethesda, MD 20892 USA NINCDS Bethesda MD USA 20892 sonance Imaging, NIH, Bethesda, MD 20892 USA Univ Minnesota, Dept Geol & Geophys, Inst Rock Magnetism, Minneapolis, MN 55455 USA Univ Minnesota Minneapolis MN USA 55455 netism, Minneapolis, MN 55455 USA
Titolo Testata:
NATURE BIOTECHNOLOGY
fascicolo: 12, volume: 19, anno: 2001,
pagine: 1141 - 1147
SICI:
1087-0156(200112)19:12<1141:MAEMLA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
MR CONTRAST AGENT; POLYAMIDOAMINE DENDRIMERS; MAMMALIAN-CELLS; GENE DELIVERY; IN-VIVO; RAT; RELAXOMETRY; GENERATION; PEPTIDE; BRAIN;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
38
Recensione:
Indirizzi per estratti:
Indirizzo: Bulte, JWM Johns Hopkins Univ, Sch Med, Dept Radiol, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 timore, MD 21205 USA
Citazione:
J.W.M. Bulte et al., "Magnetodendrimers allow endosomal magnetic labeling and in vivo tracking of stem cells", NAT BIOTECH, 19(12), 2001, pp. 1141-1147

Abstract

Magnetic resonance (MR) tracking of magnetically labeled stem and progenitor cells is an emerging technology, leading to an urgent need for magnetic probes that can make cells highly magnetic during their normal expansion inculture. We have developed magnetodendrimers as a versatile class of magnetic tags that can efficiently label mammalian cells, including human neuralstem cells (NSCs) and mesenchymal stem cells (MSCs), through a nonspecificmembrane adsorption process with subsequent intracellular (non-nuclear) localization in endosomes. The superparamagnetic iron oxide nanocomposites have been optimized to exhibit superior magnetic properties and to induce sufficient MR cell contrast at incubated doses as low as 1 mug iron/ml culturemedium. When containing between 9 and 14 pg iron/cell, labeled cells exhibit an ex vivo nuclear magnetic resonance (NMR) relaxation rate (1/T2) as high as 24-39 s(-1)/mM iron. Labeled cells are unaffected in their viability and proliferating capacity, and labeled human NSCs differentiate normally into neurons. Furthermore, we show here that NSC-derived (and LacZ-transfected), magnetically labeled oligodendroglial progenitors can be readily detected in vivo at least as long as six weeks after transplantation, with an excellent correlation between the obtained MR contrast and staining for beta -galactosidase expression. The availability of magnetodendrimers opens up the possibility of MR tracking of a wide variety of (stem) cell transplants.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 07:17:57