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Titolo:
The functional and structural outcome of inner ear gene transfer via the vestibular and cochlear fluids in mice
Autore:
Kawamoto, K; Oh, SH; Kanzaki, S; Brown, N; Raphael, Y;
Indirizzi:
Univ Michigan, Kresge Hearing Res Inst, Dept Otolaryngol Head & Neck Surg,Ann Arbor, MI 48109 USA Univ Michigan Ann Arbor MI USA 48109 & Neck Surg,Ann Arbor, MI 48109 USA Kansai Med Univ, Dept Otolaryngol, Osaka 5708506, Japan Kansai Med Univ Osaka Japan 5708506 pt Otolaryngol, Osaka 5708506, Japan Seoul Natl Univ, Coll Med, Dept Otolaryngol Head & Neck Surg, Seoul 110744, South Korea Seoul Natl Univ Seoul South Korea 110744 Surg, Seoul 110744, South Korea Keio Univ, Dept Otolaryngol, Shinjuku Ku, Tokyo 1600016, Japan Keio Univ Tokyo Japan 1600016 aryngol, Shinjuku Ku, Tokyo 1600016, Japan
Titolo Testata:
MOLECULAR THERAPY
fascicolo: 6, volume: 4, anno: 2001,
pagine: 575 - 585
SICI:
1525-0016(200112)4:6<575:TFASOO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEMICIRCULAR CANAL OCCLUSION; GUINEA-PIGS; VIRAL-ANTIGENS; IN-VIVO; PARTIAL LABYRINTHECTOMY; HEARING PRESERVATION; DEAFNESS; TRANSGENE; THERAPY; MOUSE;
Keywords:
mouse; gene transfer; adenovirus; lacZ; semicircular canal; cochleostomy; ABR; vestibular function;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Raphael, Y Univ Michigan, Kresge Hearing Res Inst, Dept Otolaryngol Head &Neck Surg,1301 E Ann St, Ann Arbor, MI 48109 USA Univ Michigan 1301 E Ann St Ann Arbor MI USA 48109 MI 48109 USA
Citazione:
K. Kawamoto et al., "The functional and structural outcome of inner ear gene transfer via the vestibular and cochlear fluids in mice", MOL THER, 4(6), 2001, pp. 575-585

Abstract

Mice present an ideal model for inner ear gene therapy because their genome is being rapidly sequenced, their generation time is relatively short, and they serve as a valuable model for human hereditary inner ear disease. However, the small size of the mouse inner ear poses a particular challenge for surgical procedures. We have developed a new approach for viral inoculation into the mature mouse inner ear, using a replication-deficient adenovirus expressing the bacterial gene lacZ. We administered the virus through the posterior semicircular canal (canalostomy) and into the cochlea (cochleostomy). Both approaches caused lacZ to be expressed in cells lining the perilymphatic space. One canalostomy case showed gene expression in sensory cells of the crista ampullaris, whereas the cochleostomy group showed gene expression in the sensory cells in the organ of Corti and saccule. Functional tests after the surgery showed that the canalostomy preserved hearing, whereas the cochleostomy did not. Any vestibular function transiently lost after the canalostomy was recovered. Our findings indicate that inoculation of adenovirus vectors into the mouse inner ear through the semicircular canal has the potential to efficiently introduce transgenes to the vestibular system and the cochlea without compromising hearing.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/02/20 alle ore 06:16:55