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Titolo:
Cyclooxygenase-2 is expressed by cumulus cells during oocyte maturation incattle
Autore:
Nuttinck, F; Reinaud, P; Tricoire, H; Vigneron, C; Peynot, N; Mialot, JP; Mermillod, P; Charpigny, G;
Indirizzi:
Ecole Natl Vet, Unite Biol Reprod, F-94704 Maisons Alfort, France Ecole Natl Vet Maisons Alfort France F-94704 4704 Maisons Alfort, France INRA, Unite Physiol Anim, Jouy En Josas, France INRA Jouy En Josas France RA, Unite Physiol Anim, Jouy En Josas, France INRA, Unite Physiol Reprod & Comportements, F-37380 Nouzilly, France INRANouzilly France F-37380 d & Comportements, F-37380 Nouzilly, France INRA, Unite Biol Dev & Biotechnol, Jouy En Josas, France INRA Jouy En Josas France Biol Dev & Biotechnol, Jouy En Josas, France
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 1, volume: 61, anno: 2002,
pagine: 93 - 101
SICI:
1040-452X(200201)61:1<93:CIEBCC>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROSTAGLANDIN-ENDOPEROXIDE SYNTHASE-2; BOVINE PREOVULATORY FOLLICLES; MESSENGER-RIBONUCLEIC-ACID; EPIDERMAL GROWTH-FACTOR; JUNCTIONAL INTERCELLULAR COMMUNICATION; LUTEINIZING-HORMONE RECEPTOR; G/H SYNTHASE-2; ESTROUS-CYCLE; OVARIAN FOLLICLES; ENDOTHELIAL-CELLS;
Keywords:
cumulus expansion; meiosis; prostaglandin-H synthase; prostanoid; EGF;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Nuttinck, F Ecole Natl Vet, Unite Biol Reprod, 7 Ave Gen De Gaulle, F-94704 Maisons Alfort, France Ecole Natl Vet 7 Ave Gen De Gaulle Maisons AlfortFrance F-94704
Citazione:
F. Nuttinck et al., "Cyclooxygenase-2 is expressed by cumulus cells during oocyte maturation incattle", MOL REPROD, 61(1), 2002, pp. 93-101

Abstract

Prostaglandins could be involved in various aspects of final differentiation of ovarian follicles. Prostaglandins are generated by the cyclooxygenase(cox) pathway. Until now, the expression pattern of isoforms cox-1 and cox-2 of cyclooxygenase in bovine cumulus-oocyte complexes (COCs) was unknown. Using immunodetection procedure, we demonstrated in the present study thatcox-2 was expressed by cumulus cells during in vivo and in vitro maturation. Time course induction of cox-2 expression was investigated during in vitro maturation using Western blot analysis. Specific signal of cox-2 was markedly evidenced from 6 hr of culture and increased to reach a maximal levelat 24 hr of culture. In vitro, cox-2 expression in COCs was associated with increased concentrations of PGE(2) and PGF(2 alpha) in the maturation medium. In addition, the effects of culture conditions on cox-2 expression wasconsidered using RT-PCR and Western-blot analysis. We demonstrated that the addition of 10 ng/ml of EGF to TCM199 clearly increased the expression level of cox-2 mRNA and protein. Higher levels of in vitro cox-2 expression was associated with greater rates of cumulus expansion and oocytes at metaphase II at 24 hr of culture. In conclusion, our present results suggest thatcox-2 expression in cumulus cells may be involved in differentiation of COCs that occurs during oocyte maturation. Mol. Reprod. Dev. 61: 93-101, 2002. (C) 2002 Wiley-Liss, Inc,

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 02:12:33