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Titolo:
Expression of spermadhesin genes in porcine male and female reproductive tracts
Autore:
Ekhlasi-Hundrieser, M; Sinowatz, F; De Wilke, IG; Waberski, D; Topfer-Petersen, E;
Indirizzi:
Hannover Sch Vet Med, Inst Reprod Med, Hannover, Germany Hannover Sch Vet Med Hannover Germany nst Reprod Med, Hannover, Germany Univ Munich, Dept Anim Anat 2, Munich, Germany Univ Munich Munich Germany iv Munich, Dept Anim Anat 2, Munich, Germany Hannover Sch Vet Med, Inst Virol, Hannover, Germany Hannover Sch Vet Med Hannover Germany ed, Inst Virol, Hannover, Germany
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 1, volume: 61, anno: 2002,
pagine: 32 - 41
SICI:
1040-452X(200201)61:1<32:EOSGIP>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
BOAR SEMINAL PLASMA; PELLUCIDA-BINDING PROTEIN; MALE GENITAL-TRACT; PSP-II GLYCOFORMS; ZONA-PELLUCIDA; IMMUNOHISTOCHEMICAL LOCALIZATION; BIOCHEMICAL-CHARACTERIZATION; STRUCTURAL CHARACTERIZATION; DISULFIDE BRIDGES; SPERM SURFACE;
Keywords:
spermadhesins; CUB domain; gene expression; cDNA sequences; seminal plasma; epididymis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Topfer-Petersen, E Tierarztl Hsch Hanover, Inst Reprod Med, Bunteweg 15, D-30559 Hannover, Germany Tierarztl Hsch Hanover Bunteweg 15 Hannover Germany D-30559
Citazione:
M. Ekhlasi-Hundrieser et al., "Expression of spermadhesin genes in porcine male and female reproductive tracts", MOL REPROD, 61(1), 2002, pp. 32-41

Abstract

Spermadhesins, secretory proteins of the male genital tract, constitute the bulk of seminal plasma proteins in pig. The nucleotide sequences of spermadhesins AWN, AQN-1, and AQN-3 from the cDNAs, derived from seminal vesicles, were determined. The coding sequences of spermadhesins revealed an overall sequence similarity of 40% at nucleotide level. Expression of spermadhesins (AWN, AQN-1, AQN-3, PSP-I, and PSP-II) in porcine male and female reproductive tracts were studied by means of RT-PCR and immunological approaches. All spermadhesins are transcribed and translated in seminal vesicles and prostate. In caudal epididymis mRNA transcripts of all spermadhesins have been detected by RT-PCR. PSP-I showed additional signals in caput epididymisand rete testis. Translation can be detected only for AWN in tissue extracts by SDS-PAGE and Western blotting. Remarkably, AWN is the only spermadhesin that is also expressed in the uterus, the uterotubal junction, and the oviduct of the female genital tract as shown by RT-PCR, cDNA-sequencing, andimmunological analysis. In sows at estrus and interestrus, in gilts and gilts 12 hr after insemination no obvious differences were noticed in the pattern of AWN-immunoreactivity in epithelial cells either of the uterotubal junction, isthmus, or ampulla. While strong staining was observed in the superficial uterine glands and in the glands of the uterotubal junction duringestrus, in diestrus this declined distinctly. The role of spermadhesins inrelation to their expression is discussed. Mot, Reprod. Dev. 61: 32-41, 2002, (C) 2002 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/11/20 alle ore 07:24:03