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Titolo:
Matrix metalloproteinase (MMP)-2 and MMP-9 and their inhibitor, TIMP-1, inhuman term decidua and fetal membranes: the effect of prostaglandin F-2 alpha and indomethacin
Autore:
Ulug, U; Goldman, S; Ben-Shlomo, I; Shalev, E;
Indirizzi:
HaEmek Med Ctr, Dept Obstet & Gynecol, Lab Res Reprod Sci, IL-18101 Afula,Israel HaEmek Med Ctr Afula Israel IL-18101 s Reprod Sci, IL-18101 Afula,Israel Technion Israel Inst Technol, Bruce Rappaport Fac Med, IL-31096 Haifa, Israel Technion Israel Inst Technol Haifa Israel IL-31096 L-31096 Haifa, Israel
Titolo Testata:
MOLECULAR HUMAN REPRODUCTION
fascicolo: 12, volume: 7, anno: 2001,
pagine: 1187 - 1193
SICI:
1360-9947(200112)7:12<1187:MM(AMA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; EXTRACELLULAR-MATRIX; GENE-EXPRESSION; PREMATURE LABOR; INDUCTION; COLLAGEN; MATRIX-METALLOPROTEINASE-9; CYCLOOXYGENASE-2; DEXAMETHASONE; FIBROBLASTS;
Keywords:
fetal membranes; indomethacin; matrix metalloproteinase; prostaglandin F-2 alpha; TIMP-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Shalev, E HaEmek Med Ctr, Dept Obstet & Gynecol, Lab Res Reprod Sci, IL-18101 Afula,Israel HaEmek Med Ctr Afula Israel IL-18101 ci, IL-18101 Afula,Israel
Citazione:
U. Ulug et al., "Matrix metalloproteinase (MMP)-2 and MMP-9 and their inhibitor, TIMP-1, inhuman term decidua and fetal membranes: the effect of prostaglandin F-2 alpha and indomethacin", MOL HUM REP, 7(12), 2001, pp. 1187-1193

Abstract

Degradation and breakdown of gestational membranes and the adjacent decidua are essential processes for the advancement of labour. We have assessed the effect of prostaglandin (PG) synthesis on the expression and activity ofmatrix metalloproteinase (MMP)-2 and MMP-9 and tissue inhibitor of metalloproteinases (TIMP-1) in fetal membranes at the edge of the placenta and decidua, by using ex-vivo organ culture of the tissues in the absence or presence of PGF(2 alpha) (0.1, 1.0 and 10 mug/ml) or a PG synthesis inhibitor, indomethacin (10(-4)-1.0(-6) mol/1). Conditioned media were assessed for MMPby zymography on gelatin containing sodium dodecyl sulphate-polyacrylamidegels and for TIMP-1 by Western blot analysis, Compared to the membranes, decidua produced significantly more MMP-2 and MMP-9 as well as TIMP-1. PGF(2alpha) caused a 2.4- and 1.9-fold increase in the production of MMP-2 and MMP-9 in the decidua, respectively (P < 0.05), and an 11.3-fold increase ofthe active form of MMP-2 (62 kDa) which could hardly be detected in basal culture conditions (P < 0.01). PGF(2 alpha) decreased TIMP-1 production by 70% in the decidua. The production of MMP-2 and MMP-9 and TIMP-1 by the amniotic and chorionic membranes was not affected by PGF(2 alpha). Indomethacin decreased the production of MMP-2 and MMP-9 by 78 and 35% in chorion, andby 70 and 58% in amnion, respectively (P < 0.05), but did not affect production in decidual tissue. Indomethacin increased the production of TIMP-1 in chorion and amnion [by, 4.1- and 4.5-fold respectively (P < 0.01)], but had no effect on decidua. Cumulatively, PGF(2 alpha) increases decidual gelatinolytic activity. Meanwhile the inhibition of PG production by indomethacin reduces total gelatinolytic activity in fetal membranes, possibly accounting for some of its labour-arresting property.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:01:02