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Titolo:
Characterization of a new brain-derived proteoglycan inhibiting retinal ganglion cell axon outgrowth
Autore:
Henke-Fahle, S; Wild, K; Sierra, A; Monnier, PP;
Indirizzi:
Univ Tubingen, Dept Ophthalmol, D-72076 Tubingen, Germany Univ Tubingen Tubingen Germany D-72076 halmol, D-72076 Tubingen, Germany Max Planck Inst Entwicklungsbiol, D-72076 Tubingen, Germany Max Planck Inst Entwicklungsbiol Tubingen Germany D-72076 ingen, Germany
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 5, volume: 18, anno: 2001,
pagine: 541 - 556
SICI:
1044-7431(200111)18:5<541:COANBP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
CHONDROITIN SULFATE PROTEOGLYCAN; TYROSINE PHOSPHATASE-ZETA/BETA; CENTRAL-NERVOUS-SYSTEM; NEURITE OUTGROWTH; MONOCLONAL-ANTIBODIES; EXTRACELLULAR-MATRIX; COLORECTAL-CANCER; CHOICE POINTS; PG-M/VERSICAN; OPTIC-NERVE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Henke-Fahle, S Univ Tubingen, Dept Ophthalmol, Schleichstr 12, D-72076 Tubingen, Germany Univ Tubingen Schleichstr 12 Tubingen Germany D-72076 many
Citazione:
S. Henke-Fahle et al., "Characterization of a new brain-derived proteoglycan inhibiting retinal ganglion cell axon outgrowth", MOL CELL NE, 18(5), 2001, pp. 541-556

Abstract

A proteoglycan was identified and isolated from physiological saline extracts of chick embryo brains by using a new monoclonal antibody (hybridoma clone mab Te38). The purified proteoglycan displayed an apparent molecular mass of 2500-3500 kDa, which became reduced to 370 and 600 kDa after digestion with chondroitinase ABC or chondroitinase AC. After additional treatment with keratanase the 600-kDa band was no longer detectable in Western blots. The specific epitope recognized by mab Te38 is an O-linked carbohydrate associated with the core protein. Tenascin-C, an extracellular matrix proteinknown to associate with several proteoglycans, copurified with the mab Te38 proteoglycan on the immunoaffinity column. Mab Te38 binds to the surface of nonneuronal cells; in sections from the primary visual system, expression is restricted to cells in the optic fissure, the dorsal optic nerve, and the chiasm. No retinal cells were found to express the mab Te38 epitope. The isolated molecule inhibited axon outgrowth from retinal explants when offered bound to a substrate consisting of either matrigel or collagen, chondroitinase treatment did not alter the inhibitory properties. The distribution and in vitro function of the Te38 proteoglycan indicate that it may servea role in guidance of retinal ganglion cell axons.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 10:13:19