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Titolo:
Association between integrin-dependent migration capacity of neural stem cells in vitro and anatomical repair following transplantation
Autore:
Prestoz, L; Relvas, JB; Hopkins, K; Patel, S; Sowinski, P; Price, J; ffrench-Constant, C;
Indirizzi:
Univ Cambridge, Dept Med Genet, Cambridge CB2 2PY, England Univ CambridgeCambridge England CB2 2PY net, Cambridge CB2 2PY, England Univ Cambridge, Cambridge Ctr Brain Repair, Cambridge CB2 2PY, England Univ Cambridge Cambridge England CB2 2PY air, Cambridge CB2 2PY, England Inst Psychiat, London SE5 8AF, England Inst Psychiat London England SE5 8AF t Psychiat, London SE5 8AF, England ReNeuron Ltd, Guildford GU2 7AF, Surrey, England ReNeuron Ltd Guildford Surrey England GU2 7AF rd GU2 7AF, Surrey, England
Titolo Testata:
MOLECULAR AND CELLULAR NEUROSCIENCE
fascicolo: 5, volume: 18, anno: 2001,
pagine: 473 - 484
SICI:
1044-7431(200111)18:5<473:ABIMCO>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
NEURONAL MIGRATION; BETA-1 INTEGRINS; CHAIN MIGRATION; ADHESION; EXPRESSION; DISTINCT; RECEPTOR; FIBRONECTIN; VITRONECTIN; HIPPOCAMPUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: ffrench-Constant, C Univ Cambridge, Dept Med Genet, Forvie Site,Robinson Way, Cambridge CB2 2PY, England Univ Cambridge Forvie Site,Robinson Way Cambridge England CB2 2PY
Citazione:
L. Prestoz et al., "Association between integrin-dependent migration capacity of neural stem cells in vitro and anatomical repair following transplantation", MOL CELL NE, 18(5), 2001, pp. 473-484

Abstract

In previous transplantation studies using neural stem cell lines immortalized by the temperature-sensitive SV40 large T-antigen, we have shown that animals with experimental hippocampal lesions resulting from four vessel occlusion recover spatial memory functions more effectively when grafted with the MHP36 cell line than with the MHP15 cell line [Gray et al. (1999). Philos. Trans. R. Soc. London Biol. Sci. 354: 1407-1421]. In the present study,we have investigated the cellular and molecular basis of these differencesin repair capacity both in vivo and in vitro. Using the same model of hippocampal damage we have shown that following transplantation MHP36 cells migrate and align within the damaged CA1 of the ipsilateral hippocampus. MHP15cells, in contrast, migrate in a more indiscriminate pattern that does notreflect the anatomy of the region. To analyze the migratory properties of these two cell lines in more detail, we performed migration assays at a nonpermissive temperature on the extracellular matrix substrates laminin, fibronectin, and vitronectin. These showed that MHP36 cells have a greater migration potential than the MHP15 cells. While the pattern of cell surface extracellular matrix receptors of the integrin family was identical in both cell lines, the different degrees of migration on vitronectin were both blocked by inhibitors of alphaV integrins. Differences in integrin signaling therefore contribute to the greater migration potential of the repairing MHP36cell line.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:01:15