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Titolo:
Investigation of seeds with high-resolution solid-state C-13 NMR
Autore:
Bardet, M; Foray, MF; Bourguignon, J; Krajewski, P;
Indirizzi:
CEA Grenoble, Serv Chim Inorgan & Biol, Dept Rech Fondamentale Mat Condensee, F-38054 Grenoble 9, France CEA Grenoble Grenoble France 9 Mat Condensee, F-38054 Grenoble 9, France CEA Grenoble, Physiol Cellulaire Vegetale Lab, Dept Biol Mol & Struct, F-38054 Grenoble, France CEA Grenoble Grenoble France F-38054 & Struct, F-38054 Grenoble, France
Titolo Testata:
MAGNETIC RESONANCE IN CHEMISTRY
fascicolo: 12, volume: 39, anno: 2001,
pagine: 733 - 738
SICI:
0749-1581(200112)39:12<733:IOSWHS>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEAR-MAGNETIC-RESONANCE; POLARIZATION TRANSFER; WOOD; CPMAS;
Keywords:
NMR; solid-state NMR; C-13 NMR; magic angle spinning; seeds; triacylglycerols; Arabidopsis thaliana; Lactuca saliva; Pisum sativum;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Bardet, M CEA Grenoble, Serv Chim Inorgan & Biol, Dept Rech Fondamentale Mat Condensee, F-38054 Grenoble 9, France CEA Grenoble Grenoble France 9 see, F-38054 Grenoble 9, France
Citazione:
M. Bardet et al., "Investigation of seeds with high-resolution solid-state C-13 NMR", MAGN RES CH, 39(12), 2001, pp. 733-738

Abstract

Magic angle spinning (MAS) C-13 NMR was employed to investigate seeds. By combining single pulse excitation (SP) and cross-polarization (cr) experiments, we showed that both liquid and solid domains of plant seeds (Arabidopsis thaliana, Lactuca sativa and Pisum sativum) can be characterized. Sharp signals in the CP/MAS spectra of seeds were attributed to triacylglycerols (TAG) belonging to the mobile phase by the use of a non-quaternary suppression (NQS) experiment. Using the CP/MAS probe designed for solid-state NMR, we demonstrated that the liquid domain of seeds could be readily analyzed under MAS conditions with classical multi-pulse experiments normally devotedto liquid-phase NMR. Such a nondestructive approach should be useful to monitor the transformation of metabolites during physiological processes and the effects of chemical molecules on seeds and other similar biological systems. Copyright (C) 2001 John Wiley & Sons, Ltd.

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Documento generato il 06/04/20 alle ore 05:36:03