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Titolo:
A single amino acid change in the nuclear localization sequence of the nsP2 protein affects the neurovirulence of Semliki Forest virus
Autore:
Fazakerley, JK; Boyd, A; Mikkola, ML; Kaariainen, L;
Indirizzi:
Univ Edinburgh, Ctr Infect Dis, Lab Clin & Mol Virol, Edinburgh EH9 1QH, Midlothian, Scotland Univ Edinburgh Edinburgh Midlothian Scotland EH9 1QH Midlothian, Scotland Univ Helsinki, Inst Biotechnol, Helsinki, Finland Univ Helsinki HelsinkiFinland inki, Inst Biotechnol, Helsinki, Finland
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 1, volume: 76, anno: 2002,
pagine: 392 - 396
SICI:
0022-538X(200201)76:1<392:ASAACI>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
CENTRAL-NERVOUS-SYSTEM; NONSTRUCTURAL PROTEIN-NSP2; NUCLEOTIDE-SEQUENCE; VIRULENT-STRAIN; INFECTION; MICE; AVIRULENT; BRAIN; CELLS; DEMYELINATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Fazakerley, JK Univ Edinburgh, Ctr Infect Dis, Lab Clin & Mol Virol, Summerhall, Edinburgh EH9 1QH, Midlothian, Scotland Univ Edinburgh Summerhall Edinburgh Midlothian Scotland EH9 1QH
Citazione:
J.K. Fazakerley et al., "A single amino acid change in the nuclear localization sequence of the nsP2 protein affects the neurovirulence of Semliki Forest virus", J VIROLOGY, 76(1), 2002, pp. 392-396

Abstract

The replicase protein nsP2 of Semliki Forest virus (SFV) has a (RRR)-R-648nuclear localization signal and is transported to the nucleus. SFV-RDR hasa single amino acid change which disrupts this sequence and nsP2 nuclear transport. In BHK cells, SFV4 and SFV-RDR replicate to high titers, but SFV-RDR is less virulent in mice. We compared the replication of SFV4 and SFV-RDR in adult mouse brain. Both SFV4 and SFV-RDR were neuroinvasive followingintraperitoneal inoculation. SFV4 spread rapidly throughout the brain, whereas SFV-RDR infection was confined to small foci of cells. Both viruses infected neurons and oligodendrocytes. Both viruses induced apoptosis in cultured BHK cells but not in the cells of the adult mouse brain. SFV-RDR infection of mice lacking alpha/beta interferon receptors resulted in widespreadvirus distribution in the brain. Thus, a component of the viral replicase plays an important role in the neuropathogenesis of SFV.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 12:25:49