Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Adenovirus hexon protein is a potent adjuvant for activation of a cellularimmune response
Autore:
Molinier-Frenkel, V; Lengagne, R; Gaden, F; Hong, SS; Choppin, J; Gahery-Segard, H; Boulanger, P; Guillet, JG;
Indirizzi:
Hop Cochin, Inst Cochin Genet Mol, INSERM U445, Lab Immunol Pathol Infect & Tumorales, F-75014 Paris, France Hop Cochin Paris France F-75014 nfect & Tumorales, F-75014 Paris, France Fac Med RTH Laennec, CNRS UMR 5537, Lab Virol & Pathogenese Mol, F-69008 Lyon, France Fac Med RTH Laennec Lyon France F-69008 genese Mol, F-69008 Lyon, France
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 1, volume: 76, anno: 2002,
pagine: 127 - 135
SICI:
0022-538X(200201)76:1<127:AHPIAP>2.0.ZU;2-0
Fonte:
ISI
Lingua:
ENG
Soggetto:
CYTOTOXIC T-LYMPHOCYTES; NF-KAPPA-B; DENDRITIC CELLS; GENE-THERAPY; RECOMBINANT ADENOVIRUS; PENTON-BASE; IN-VIVO; CAPSID COMPONENTS; INFECTED-CELLS; BALB/C MICE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
48
Recensione:
Indirizzi per estratti:
Indirizzo: Molinier-Frenkel, V Hop Cochin, Inst Cochin Genet Mol, INSERM U445, Lab Immunol Pathol Infect & Tumorales, 27 Rue Faubourg St Jacques, F-75014 Paris,France Hop Cochin 27 Rue Faubourg St Jacques Paris France F-75014
Citazione:
V. Molinier-Frenkel et al., "Adenovirus hexon protein is a potent adjuvant for activation of a cellularimmune response", J VIROLOGY, 76(1), 2002, pp. 127-135

Abstract

The capacity of recombinant adenoviruses (rAd) to induce immunization against their transgene products has been well documented. In the present study, we evaluated the vaccinal adjuvant role of rAd independently of its vector function. BALB/c mice received one subcutaneous injection of a mixture ofsix lipopeptides (LP6) used as a model immunogen, along with AdE1 degrees (10(9) particles), a first-generation rAd empty vector. Although coinjectedwith a suboptimal dose of lipopeptides, AdE1 degrees significantly improved the effectiveness of the vaccination, even in the absence of booster immunization. In contrast to mice that received LP6 alone or LP6 plus a mock adjuvant, mice injected with AdE1 degrees plus LP6 developed both a polyspecific T-helper type I response and an effector CD8 T-cell response specific to at least two class I-restricted epitopes. The helper response was still observed when immunization was performed using LP6 plus a mixture of solublecapsid components released from detergent-disrupted virions. When mice were immunized with LP6 and each individual capsid component, i.e., hexon, penton base, or fiber, the results obtained suggested that hexon protein was responsible for the adjuvant effect exerted by disrupted Ad particles on thehelper response to the immunogen. Our results thus have some important implications not only in vaccinology but also for gene therapy using rAd vectors.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 19:19:53