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Titolo:
Group A rotavirus infection and age-dependent diarrheal disease in rats: Anew animal model to study the pathophysiology of rotavirus infection
Autore:
Ciarlet, M; Conner, ME; Finegold, MJ; Estes, MK;
Indirizzi:
Baylor Coll Med, Dept Mol Virol & Microbiol, Houston, TX 77030 USA Baylor Coll Med Houston TX USA 77030 l & Microbiol, Houston, TX 77030 USA Vet Affairs Med Ctr, Houston, TX 77030 USA Vet Affairs Med Ctr Houston TXUSA 77030 s Med Ctr, Houston, TX 77030 USA Texas Childrens Hosp, Dept Pathol, Houston, TX 77030 USA Texas Childrens Hosp Houston TX USA 77030 t Pathol, Houston, TX 77030 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 1, volume: 76, anno: 2002,
pagine: 41 - 57
SICI:
0022-538X(200201)76:1<41:GARIAA>2.0.ZU;2-D
Fonte:
ISI
Lingua:
ENG
Soggetto:
REO-LIKE VIRUS; HETEROLOGOUS ROTAVIRUS; PROTECTIVE IMMUNITY; RHESUS ROTAVIRUS; RABBIT MODEL; NONSTRUCTURAL GLYCOPROTEIN; INTESTINAL MORPHOLOGY; PATHOLOGICAL-CHANGES; MURINE ROTAVIRUS; MOUSE MODEL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
79
Recensione:
Indirizzi per estratti:
Indirizzo: Estes, MK Baylor Coll Med, Dept Mol Virol & Microbiol, 1 Baylor Plaza,MailStop BCM-385, Houston, TX 77030 USA Baylor Coll Med 1 Baylor Plaza,Mail Stop BCM-385 Houston TX USA 77030
Citazione:
M. Ciarlet et al., "Group A rotavirus infection and age-dependent diarrheal disease in rats: Anew animal model to study the pathophysiology of rotavirus infection", J VIROLOGY, 76(1), 2002, pp. 41-57

Abstract

Group A rotaviruses are major pathogens causing acute gastroenteritis in children and animals. To determine if group A rotavirus replicates and induces disease in rats, antibody-negative Lewis neonatal or adult rats were inoculated orally with tissue culture-adapted human (Wa, W161, and HAL1166), simian (rhesus rotavirus [RRV] and SA11), bovine (WC3), lapine (ALA), or porcine (OSU) rotavirus strains, wild-type murine (ECwt) rotavirus strain, or phosphate-buffered saline (PBS). Rotavirus infection in rats was evaluated by (i) clinical findings, (ii) virus antigen shedding or infectious virus titers in the feces or intestinal contents measured by enzyme-linked immunosorbent assay or fluorescent-focus assay, (iii) histopathological changes inthe small intestine, (iv) distribution of rotavirus antigen in small-intestine sections by immunofluorescence, and (v) growth rate. Rotavirus infection of 5-day-old but not greater than or equal to 21-day-old rats resulted in diarrhea that lasted from I to 10 days postinoculation. The severity of disease and spread of infection to naive littermates differed depending on the virus strain used for inoculation. The duration of virus antigen shedding following infection was considerably prolonged (up to 10 days) in neonatal rats compared to that in 21-day-old rats (1 or 2 days). Based on lack of virus antigen shedding and disease induction, the murine ECwt rotavirus wasthe only strain tested that did not infect rats. Histopathological changesin the small-intestine mucosa of 5-day-old RRV-inoculated rats but not of PBS-inoculated rats was limited to extensive enterocyte vacuolation in the ileum. In RRV-inoculated neonatal rats, rotavirus antigen was detected in the epithelial cells on the upper half of the intestinal villi of the jejunum and ileum. In addition, infection of neonatal rats with RRV but not with PBS resulted in reduced weight gain. Rats infected with group A rotavirusesprovide a new animal model with unique features amenable to investigate rotavirus pathogenesis and the molecular mechanisms of intestinal development, including physiological factors that may regulate age-dependent rotavirus-induced diarrhea.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 07:36:34