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Titolo:
Molecular epidemiology of human immunodeficiency virus type 1 transmissionin a heterosexual cohort of discordant couples in Zambia
Autore:
Trask, SA; Derdeyn, CA; Fideli, U; Chen, YL; Meleth, S; Kasolo, F; Musonda, R; Hunter, E; Gao, F; Allen, S; Hahn, BH;
Indirizzi:
Univ Alabama, Sch Med, Dept Med, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 , Dept Med, Birmingham, AL 35294 USA Univ Alabama, Sch Med, Dept Microbiol, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 Microbiol, Birmingham, AL 35294 USA Univ Alabama, Sch Publ Hlth, Dept Epidemiol & Int Hlth, Birmingham, AL 35294 USA Univ Alabama Birmingham AL USA 35294 & Int Hlth, Birmingham, AL 35294 USA Univ Lusaka, Teaching Hosp, Dept Pathol & Microbiol, Lusaka, Zambia Univ Lusaka Lusaka Zambia Hosp, Dept Pathol & Microbiol, Lusaka, Zambia Trop Dis Res Ctr, Ndola, Zambia Trop Dis Res Ctr Ndola ZambiaTrop Dis Res Ctr, Ndola, Zambia
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 1, volume: 76, anno: 2002,
pagine: 397 - 405
SICI:
0022-538X(200201)76:1<397:MEOHIV>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEOTIDE-SEQUENCE ANALYSIS; FULL-LENGTH CLONES; SUBTYPE-C; HIV TYPE-1; GLENOCHIL PRISON; GENOMES; RECOMBINATION; DIVERSITY; OUTBREAK; AFRICA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Hahn, BH Univ Alabama, Sch Med, Dept Med, 720 20th St S,KAUL 816, Birmingham, AL 35294 USA Univ Alabama 720 20th St S,KAUL 816 Birmingham AL USA 35294 4 USA
Citazione:
S.A. Trask et al., "Molecular epidemiology of human immunodeficiency virus type 1 transmissionin a heterosexual cohort of discordant couples in Zambia", J VIROLOGY, 76(1), 2002, pp. 397-405

Abstract

Most human immunodeficiency virus type 1 (HIV-1) transmissions in sub-Saharan Africa are believed to occur between married adults who are discordant for their HIV-1 infection status; however, no studies to date have investigated the molecular epidemiology of such transmission events. Here we reportthe genetic characterization of HIV-1 strains from 149 transmission pairs that were identified prospectively in a cohort of discordant couples in Lusaka, Zambia. Subgenomic gag, gp120, gp41, and/or long terminal repeat regions were amplified by PCR analysis of uncultured blood samples from both partners and sequenced without interim cloning. Pairwise genetic distances were calculated for the regions analyzed and compared to those of subtype-specific reference sequences as well as local controls. Sequence relationships were also examined by phylogenetic tree analysis. By these approaches, epidemiological linkage was established for the majority of transmission pairs. Viruses from 129 of the 149 couples (87%) were very closely related and clustered together in phylogenetic trees in a statistically highly significant manner. In contrast, viruses from 20 of the 149 couples (13%) were only distantly related in two independent genomic regions, thus ruling out transmission between the two partners. The great majority (95%) of transmitted viruses were of subtype C origin, although representatives of subtypes A, D, G, and J were also identified. There was no evidence for extensive transmission networks within the cohort, although two phylogenetic subclusters of viruses infecting two couples each were identified. Taken together, these data indicate that molecular epidemiological analyses of presumed transmission pairs are both feasible and required to determine behavioral, virological, and immunological correlates of heterosexual transmission in sub-Saharan Africa with a high level of accuracy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 17:39:12