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Titolo:
A nucleocapsid functionality contained within the amino terminus of the Ty1 protease that is distinct and separable from proteolytic activity
Autore:
Lawler, JF; Merkulov, GV; Boeke, JD;
Indirizzi:
Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, Baltimore, MD 21205 USA Johns Hopkins Univ Baltimore MD USA 21205 Genet, Baltimore, MD 21205 USA
Titolo Testata:
JOURNAL OF VIROLOGY
fascicolo: 1, volume: 76, anno: 2002,
pagine: 346 - 354
SICI:
0022-538X(200201)76:1<346:ANFCWT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
VIRUS-LIKE PARTICLES; SACCHAROMYCES-CEREVISIAE GENOME; HELPER COMPONENT-PROTEINASE; YEAST RETROTRANSPOSON TY1; ELEMENT TRANSPOSITION; REVERSE-TRANSCRIPTASE; TRANSFER-RNA; SPT3 GENE; BINDING; SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
42
Recensione:
Indirizzi per estratti:
Indirizzo: Boeke, JD Johns Hopkins Univ, Sch Med, Dept Mol Biol & Genet, 725 N Wolfe St, Baltimore, MD 21205 USA Johns Hopkins Univ 725 N Wolfe St Baltimore MD USA 21205 205 USA
Citazione:
J.F. Lawler et al., "A nucleocapsid functionality contained within the amino terminus of the Ty1 protease that is distinct and separable from proteolytic activity", J VIROLOGY, 76(1), 2002, pp. 346-354

Abstract

Ty1 is the most successful of the five endogenous yeast retrotransposons. The life cycle of Ty1 dictates that a number of nucleocapsid (NC)-facilitated events occur although the protein(s) responsible for these events has not been identified. The positioning of the NC peptide is conserved at the carboxy terminus of the Gag protein among most long terminal repeat (LTR)-containing retroelements. An analogous region of Ty1 that simultaneously encodes part of Gag, protease (PR), and the C-terminal p4 peptide was mutagenized. Some of these mutations result in smaller-than-normal virus-like particles (VLPs). The mutants were also found to impair an NC-like functionality contained within the amino terminus of the protease that is distinct and separable from its proteolytic activity. Remarkably, these mutants have distinct defects in reverse transcription.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 19:10:27