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Titolo:
Canine parentage testing based on microsatellite polymorphisms
Autore:
Ichikawa, Y; Takagi, K; Tsumagari, S; Ishihama, K; Morita, M; Kanemaki, M; Takeishi, M; Takahashi, H;
Indirizzi:
Nihon Univ, Coll Bioresource Sci, Dept Vet Med, Lab Vet Obstet & Gynecol, Fujisawa, Kanagawa 2528510, Japan Nihon Univ Fujisawa Kanagawa Japan 2528510 isawa, Kanagawa 2528510, Japan Livestock Improvement Assoc Japan, Livestock Improvement Technol Lab, Maebashi, Gumma 3710121, Japan Livestock Improvement Assoc Japan Maebashi Gumma Japan 3710121 121, Japan
Titolo Testata:
JOURNAL OF VETERINARY MEDICAL SCIENCE
fascicolo: 11, volume: 63, anno: 2001,
pagine: 1209 - 1213
SICI:
0916-7250(200111)63:11<1209:CPTBOM>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
MARKERS; DOG; REPEATS; GENOME;
Keywords:
canine; DNA; microsatellite; parentage testing;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
12
Recensione:
Indirizzi per estratti:
Indirizzo: Ichikawa, Y Nihon Univ, Coll Bioresource Sci, Dept Vet Med, Lab Vet Obstet& Gynecol, Fujisawa, Kanagawa 2528510, Japan Nihon Univ Fujisawa KanagawaJapan 2528510 awa 2528510, Japan
Citazione:
Y. Ichikawa et al., "Canine parentage testing based on microsatellite polymorphisms", J VET MED S, 63(11), 2001, pp. 1209-1213

Abstract

To establish an accurate method for parentage testing in dogs, microsatellite DNA repeat length polymorphisms were examined. We selected twenty microsatellite markers reported previously and examined their application for parentage testing in Beagles and Labrador Retrievers. Heterozygosity (He), Polymorphism Information Content (PIC), the probabilities of Paternity Exclusion (PE) and the combined PE were calculated from allelic frequencies of the markers. All markers amplified by polymerase chain reactions were polymorphic and many markers showed high He and PIC in the both breeds. The final combined PEs in Beagles and Labrador Retrievers were 0.999994 and 0.999920,respectively. The results suggest that the twenty markers can be applied for routine parentage testing in dogs.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:31:26