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Titolo:
Toxic effect of L-2-chloropropionate on cultured rat cerebellar granule cells is ameliorated after inhibition of reactive oxygen species formation
Autore:
Myhre, O; Bjugan, B; Fonnum, F;
Indirizzi:
Norwegian Def Res Estab, Div Protect & Mat, N-2027 Kjeller, Norway Norwegian Def Res Estab Kjeller Norway N-2027 at, N-2027 Kjeller, Norway
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 5, volume: 66, anno: 2001,
pagine: 992 - 997
SICI:
0360-4012(200112)66:5<992:TEOLOC>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
MITOCHONDRIAL PERMEABILITY TRANSITION; 2-CHLOROPROPIONIC ACID; CORTICAL-NEURONS; NITRIC-OXIDE; IN-VIVO; NECROSIS; INVOLVEMENT; GLUTATHIONE; ACTIVATION; RELEVANCE;
Keywords:
free radicals; cell death; L-2-chloropropionate;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Myhre, O Norwegian Def Res Estab, Div Protect & Mat, POB 25, N-2027 Kjeller, Norway Norwegian Def Res Estab POB 25 Kjeller Norway N-2027 ler, Norway
Citazione:
O. Myhre et al., "Toxic effect of L-2-chloropropionate on cultured rat cerebellar granule cells is ameliorated after inhibition of reactive oxygen species formation", J NEUROSC R, 66(5), 2001, pp. 992-997

Abstract

Oral administration of rats to L-2-chloropropionate (L-CPA) causes selective necrosis to the granule cell layer of the cerebellum in vivo and to cultured rat cerebellar granule cells in vitro. The present study was conductedto characterize the involvement of reactive oxygen species (ROS) in cell death Of L-CPA to rat cerebellar granule cells in vitro. Exposure to L-CPA (0.625-10 mM) produced a concentration dependent increase in formation of 2,7-dichlorofluorescein (DCF) as a measure of formation of ROS. The elevationof ROS was inhibited after incubation of the cells with the ERK-type of MAP kinases inhibitor U0126, the mitochondrial permeability transition pore inhibitor cyclosporin A (CSA), the antioxidant vitamin E, and the spin trap N-tert-butyl-alpha-(2-sulfophenyl)-nitrone (s-PBN). Measurements of nitrite(NO2) in the cell culture supernatant using the Griess reagent indicate generation of nitric oxide (NO) after exposure to L-CPA. Incubation with L-CPA (10 mM) for 48 hr lead to cell death (90%). When the granule cells were incubated with L-CPA in combination with the inhibitors of free radical production, the cell death was ameliorated. The results show that L-CPA is toxic to granular cells by production of ROS. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 04:43:54