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Titolo:
Differential expression of glutamate dehydrogenase in cultured neurons andastrocytes from mouse cerebellum and cerebral cortex
Autore:
Zaganas, I; Waagepetersen, HS; Georgopoulos, P; Sonnewald, U; Plaitakis, A; Schousboe, A;
Indirizzi:
Royal Danish Sch Pharm, Dept Pharmacol, Neurosci Pharmabiotec Res Ctr, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm Copenhagen Denmark DK-2100 00 Copenhagen, Denmark Univ Crete, Dept Neurol, Sch Hlth Sci, Iraklion, Greece Univ Crete Iraklion Greece Dept Neurol, Sch Hlth Sci, Iraklion, Greece Norwegian Univ Sci & Technol, Dept Clin Neurosci, Trondheim, Norway Norwegian Univ Sci & Technol Trondheim Norway urosci, Trondheim, Norway
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 5, volume: 66, anno: 2001,
pagine: 909 - 913
SICI:
0360-4012(200112)66:5<909:DEOGDI>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
ONTOGENETIC DEVELOPMENT; METABOLIZING ENZYMES; AMINO-ACID; RAT; BRAIN; INTERNEURONS; ISOPROTEINS; RELEASE; INVIVO;
Keywords:
alpha-ketoglutarate; ammonia; GABAergic neurons; glutamatergic neurons;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
29
Recensione:
Indirizzi per estratti:
Indirizzo: Schousboe, A Royal Danish Sch Pharm, Dept Pharmacol, Neurosci PharmabiotecRes Ctr, 2 Univ Pk, DK-2100 Copenhagen, Denmark Royal Danish Sch Pharm 2 Univ Pk Copenhagen Denmark DK-2100
Citazione:
I. Zaganas et al., "Differential expression of glutamate dehydrogenase in cultured neurons andastrocytes from mouse cerebellum and cerebral cortex", J NEUROSC R, 66(5), 2001, pp. 909-913

Abstract

Glutamate dehydrogenase (GDH) specific activities, kinetic properties and allosteric regulation were studied in extracts from cultured neurons and astrocytes prepared from mouse cerebral cortex and cerebellum. Considerable differences were observed in the specific activity of the enzyme among the different cell types with astrocytes expressing the highest GDH activity. This may reflect the functional importance of these cells in glutamate uptakeand metabolism. Among the neurons, the glutamatergic cerebellar granule cells showed a GDH specific activity that was 60% higher (P < 0.01) than thatof the GABAergic cerebral cortical neurons. Also, the K-m for ammonia was 1.7-fold higher in the cortical neurons than in the other cell types. Thesefindings may reflect a particular need for the glutamatergic granule cellsto synthesize glutamate via the GDH pathway. No differences were observed among the different cell types with regard to the allosteric properties of GDH expressed by these cells. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/01/20 alle ore 01:28:26