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Titolo:
alpha-cyano-4-hydroxycinnamate decreases both glucose and lactate metabolism in neurons and astrocytes: Implications for lactate as an energy substrate for neurons
Autore:
McKenna, MC; Hopkins, IB; Carey, A;
Indirizzi:
Univ Maryland, Sch Med, Dept Pediat, Baltimore, MD 21201 USA Univ Maryland Baltimore MD USA 21201 Dept Pediat, Baltimore, MD 21201 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 5, volume: 66, anno: 2001,
pagine: 747 - 754
SICI:
0360-4012(200112)66:5<747:ADBGAL>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-BRAIN ASTROCYTES; HIPPOCAMPAL SLICE PREPARATION; CORTICAL SYNAPTIC TERMINALS; IN-VITRO; GLUTAMATE METABOLISM; TRANSPORTER PROTEINS; RECOVERY; CELLS; ACID; MITOCHONDRIAL;
Keywords:
alpha-cyano-4-hydroxycinnamate; glucose; lactate; astrocytes; neurons; energy metabolism;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
65
Recensione:
Indirizzi per estratti:
Indirizzo: McKenna, MC Univ Maryland, Sch Med, Dept Pediat, 10-035 BRB,655 W Baltimore, Baltimore, MD 21201 USA Univ Maryland 10-035 BRB,655 W Baltimore Baltimore MD USA 21201
Citazione:
M.C. McKenna et al., "alpha-cyano-4-hydroxycinnamate decreases both glucose and lactate metabolism in neurons and astrocytes: Implications for lactate as an energy substrate for neurons", J NEUROSC R, 66(5), 2001, pp. 747-754

Abstract

The rates of uptake and oxidation of [U-C-14] lactate and [U-C-14]glucose were determined in primary cultures of astrocytes and neurons from rat brain, in the presence and absence of the monocarboxylic acid transport inhibitor alpha -cyano-4-hydroxycinnamate (4-CIN). The rates of uptake for 1 mM lactate and glucose were 7.45 +/- 1.35 and 8.80 +/- 1.0 nmol/30 sec/mg protein in astrocytes and 2.36 +/- 0.19 and 1.93 +/- 0.16 nmol/30 sec/mg protein in neuron cultures, respectively. Lactate transport into both astrocytes and neurons was significantly decreased by 0.25-1.0 mM 4-CIN; however, glucose uptake was not affected. The rates of (CO2)-C-14 formation from 1 mM lactate and glucose were 12.49 +/- 0.77 and 3.42 +/- 0.67 nmol/ hr/mg protein in astrocytes and 29.32 +/- 2.81 and 10.04 +/- 1.79 nmol/hr/mg protein in neurons, respectively. Incubation with 0.25 mM 4-CIN decreased the oxidation of lactate and glucose to 57.1% and 54.1% of control values in astrocytes and to 13.2% and 41.6% of the control rates in neurons, respectively. Preincubation with 4-CIN further decreased the oxidation of both glucose and lactate. Studies with glucose specifically labeled in the one and six positionsdemonstrated that 4-CIN decreased mitochondrial glucose oxidation but did not impair the metabolism of glucose via the pentose phosphate pathway in the cytosol. The lack of effect of 4-CIN on glutamate oxidation demonstratedthat overall mitochondrial metabolism was not impaired. These findings suggest that the impaired neuronal function and tissue damage in the presence of 4-CIN observed in other studies may be due in part to decreased uptake of lactate; however, the effects of 4-CIN on mitochondrial transport would significantly decrease the oxidative metabolism of pyruvate derived from both glucose and lactate. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/02/20 alle ore 05:24:07