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Titolo:
Functional relevance of carnitine transporter OCTN2 to brain distribution of L-carnitine and acetyl-L-carnitine across the blood-brain barrier
Autore:
Kido, Y; Tamai, I; Ohnari, A; Sai, Y; Kagami, T; Nezu, J; Nikaido, H; Hashimoto, N; Asano, M; Tsuji, A;
Indirizzi:
Kanazawa Univ, Fac Pharmaceut Sci, Dept Pharmacol Dynam, Kanazawa, Ishikawa 9200934, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9200934 wa, Ishikawa 9200934, Japan Japan Sci & Technol Corp, CREST, Kawaguchi, Japan Japan Sci & Technol Corp Kawaguchi Japan Corp, CREST, Kawaguchi, Japan Chugai Res Inst Mol Med Inc, Ibaraki, Osaka, Japan Chugai Res Inst Mol MedInc Ibaraki Osaka Japan c, Ibaraki, Osaka, Japan Kanazawa Univ, Fac Med, Inst Expt Anim, Kanazawa, Ishikawa 9200934, Japan Kanazawa Univ Kanazawa Ishikawa Japan 9200934 wa, Ishikawa 9200934, Japan
Titolo Testata:
JOURNAL OF NEUROCHEMISTRY
fascicolo: 5, volume: 79, anno: 2001,
pagine: 959 - 969
SICI:
0022-3042(200112)79:5<959:FROCTO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ORGANIC CATION TRANSPORTER; MONOCARBOXYLIC ACIDS; TISSUE DISTRIBUTION; IN-VITRO; CELLS; CLONING;
Keywords:
acetyl-L-carnitine; blood-brain barrier; jvs mice; L-carnitine; octn2; transport;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Tsuji, A Kanazawa Univ, Fac Pharmaceut Sci, Dept Pharmacol Dynam, 13-1 Takara Machi, Kanazawa, Ishikawa 9200934, Japan Kanazawa Univ 13-1 Takara Machi Kanazawa Ishikawa Japan 9200934 n
Citazione:
Y. Kido et al., "Functional relevance of carnitine transporter OCTN2 to brain distribution of L-carnitine and acetyl-L-carnitine across the blood-brain barrier", J NEUROCHEM, 79(5), 2001, pp. 959-969

Abstract

Transport of L-[H-3]carnitine and acetyl-L-[H-3]carnitine at the blood-brain barrier (BBB) was examined by using in vivo and in vitro models. In vivobrain uptake of acetyl-L-[H-3]carnitine, determined by a rat brain perfusion technique, was decreased in the presence of unlabeled acetyl-L-carnitineand in the absence of sodium ions. Similar transport properties for L-[H-3]carnitine and/or acetyl-L-[H-3]carnitine were observed in primary culturedbrain capillary endothelial cells (BCECs) of rat, mouse, human, porcine and bovine, and immortalized rat BCECs, RBEC1. Uptakes of L-[H-3]carnitine and acetyl-L[H-3]carnitine by RBEC1 were sodium ion-dependent, saturable withK-m values of 33.1 +/- 11.4 mum and 31.3 +/- 11.6 mum, respectively, and inhibited by carnitine analogs. These transport properties are consistent with those of carnitine transport by OCTN2. OCTN2 was confirmed to be expressed in rat and human BCECs by an RT-PCR method. Furthermore, the uptake of acetyl-L-[H-3]carnitine by the BCECs of juvenile visceral steatosis (jvs) mouse, in which OCTN2 is functionally defective owing to a genetical missensemutation of one amino acid residue, was reduced. The brain distributions of L-[H-3]carnitine and acetyl-L-[H-3]carnitine in jvs mice were slightly lower than those of wild-type mice at 4 h after intravenous administration. These results suggest that OCTN2 is involved in transport of L-carnitine andacetyl-L-carnitine from the circulating blood to the brain across the BBB.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 22:18:29