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Titolo:
Characterization of individual adenine residues in DNA by a combination ofsite-selective C8-deuteration and UV resonance Raman difference spectroscopy
Autore:
Toyama, A; Miyagawa, Y; Yoshimura, A; Fujimoto, N; Takeuchi, H;
Indirizzi:
Tohoku Univ, Grad Sch Pharmaceut Sci, Sendai, Miyagi 9808578, Japan TohokuUniv Sendai Miyagi Japan 9808578 ci, Sendai, Miyagi 9808578, Japan
Titolo Testata:
JOURNAL OF MOLECULAR STRUCTURE
fascicolo: 1, volume: 598, anno: 2001,
pagine: 85 - 91
SICI:
0022-2860(20011031)598:1<85:COIARI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
HYDROGEN-BONDING STATES; DRUG ACTINOMYCIN-D; TETRANUCLEOTIDE SEQUENCES; BINDING SPECIFICITIES; CRYSTAL-STRUCTURE; SPECTRA; RING; FREQUENCIES; ADENOSINE; COMPLEX;
Keywords:
site-selective isotope labeling; UV resonance Raman spectroscopy; adenine residue; actinomycin D;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Takeuchi, H Tohoku Univ, Grad Sch Pharmaceut Sci, Sendai, Miyagi 9808578, Japan Tohoku Univ Sendai Miyagi Japan 9808578 Miyagi 9808578, Japan
Citazione:
A. Toyama et al., "Characterization of individual adenine residues in DNA by a combination ofsite-selective C8-deuteration and UV resonance Raman difference spectroscopy", J MOL STRUC, 598(1), 2001, pp. 85-91

Abstract

Raman spectroscopy has been combined with site-selective isotopic labelingtechniques to obtain structural information on a selected nucleic acid residue in oligonucleotides. In the difference spectrum between the unlabeled and site-selectively labeled oligonucleotides, the Raman signals from the residue at the labeled position show up, while the signals from the residuesat unlabeled positions are canceled out. To demonstrate the utility of this new method, we have prepared a self-complementary tetradecamer DNA, d(AGTGCTCGAGCACT)(2), containing single C8-deuterated adenine at position 9 (A9)or 12 (A12). UV (257 nm) resonance Raman difference spectra between the unlabeled and labeled oligonucleotides in solution reveal slightly different microenvironments around A9 and A 12 as expected for a double-stranded helical structure of the tetradecamer DNA. In the presence of an antitumor antibiotic, actinomycin D, which intercalates into the 5'-GC-3' sequence of DNA, the Raman signals of A9 on the 5'-side of the intercalation site become significantly weaker, indicating an increased base stacking with adjacent guanine bases. In contrast, the Raman signals of A12 on the 3'-side are not affected by the binding of actinomycin D. This observation provides the first experimental evidence that the intercalation of actinomycin D induces an asymmetric structural alteration of DNA in solution. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 19/09/20 alle ore 12:40:17