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Titolo:
Classification and phylogenetic analysis of the cAMP-dependent protein kinase regulatory subunit family
Autore:
Canaves, JM; Taylor, SS;
Indirizzi:
Univ Calif San Diego, Dept Chem & Biochem, La Jolla, CA 92093 USA Univ Calif San Diego La Jolla CA USA 92093 iochem, La Jolla, CA 92093 USA
Titolo Testata:
JOURNAL OF MOLECULAR EVOLUTION
fascicolo: 1, volume: 54, anno: 2002,
pagine: 17 - 29
SICI:
0022-2844(200201)54:1<17:CAPAOT>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
AMINO-ACID SUBSTITUTION; GENE ACTIVATOR PROTEIN; BOVINE CARDIAC-MUSCLE; BINDING-SITES; CYCLIC-AMP; DICTYOSTELIUM-DISCOIDEUM; EVOLUTIONARY IMPLICATIONS; SACCHAROMYCES-CEREVISIAE; NEW-GENERATION; SEQUENCE;
Keywords:
PKA; cAMP; cAPK; regulatory subunit; motif classification; phylogeny; signature;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
51
Recensione:
Indirizzi per estratti:
Indirizzo: Taylor, SS Univ Calif San Diego, Dept Chem & Biochem, 0654,9500 Gilman Dr,La Jolla, CA 92093 USA Univ Calif San Diego 0654,9500 Gilman Dr La Jolla CA USA 92093
Citazione:
J.M. Canaves e S.S. Taylor, "Classification and phylogenetic analysis of the cAMP-dependent protein kinase regulatory subunit family", J MOL EVOL, 54(1), 2002, pp. 17-29

Abstract

The members of the PKA regulatory subunit family (PKA-R family) were analyzed by multiple sequence alignment and clustering based on phylogenetic tree construction. According to the phylogenetic trees generated from multiplesequence alignment of the complete sequences, the PKA-R family was dividedinto four subfamilies (types I to IV). Members of each subfamily were exclusively from animals (types I and II), fungi (type III), and alveolates (type IV). Application of the same methodology to the cAMP-binding domains, and subsequently to the region delimited by beta -strands 6 and 7 of the crystal structures of bovine RI alpha and rat RII beta (the phosphate-binding cassette; PBC), proved that this highly conserved region was enough to classify unequivocally the members of the PKA-R family. A single signature sequence, F-G-E-[LIV]-A-L-[LIMV]-x(3)[PV]-R-[ANQV]-A. corresponding to the PBC was identified which is characteristic of the PKA-R family and is sufficientto distinguish it from other members of the cyclic nucleotide-binding protein superfamily. Specific determinants for the A and B domains of each R-subunit type were also identified. Conserved residues defining the signature motif are important for interaction with cAMP or for positioning the residues that direct ! interact with cAMP. Conversely, residues that define subfamilies or domain types are not conserved and are mostly located on the loopthat connects alpha -helix B' an beta strand 7.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:02:14