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Titolo:
Secretory activity of bovine ovarian granulosa cells transfected with sense and antisense insulin-like growth factor (IGF) binding protein-3 and the response to IGF-I, GH, LH, oxytocin and oestradiol
Autore:
Sirotkin, AV; Makarevich, AV; Corkins, MR; Kotwica, J; Kwon, HB; Bulla, J; Hetenyi, L;
Indirizzi:
Res Inst Anim Prod, Lab Endocrinol, Nitra 94992, Slovakia Res Inst Anim Prod Nitra Slovakia 94992 ndocrinol, Nitra 94992, Slovakia Indiana Univ, James Whitcomb Riley Hosp Children, Indianapolis, IN 46202 USA Indiana Univ Indianapolis IN USA 46202 ildren, Indianapolis, IN 46202 USA Inst Anim Prod & Food Res, PL-10718 Olszytn Kortowo, Poland Inst Anim Prod& Food Res Olszytn Kortowo Poland PL-10718 ortowo, Poland Chonnam Natl Univ, Dept Biol, Kwangju 500753, South Korea Chonnam Natl Univ Kwangju South Korea 500753 Kwangju 500753, South Korea
Titolo Testata:
JOURNAL OF MOLECULAR ENDOCRINOLOGY
fascicolo: 3, volume: 27, anno: 2001,
pagine: 329 - 338
SICI:
0952-5041(200112)27:3<329:SAOBOG>2.0.ZU;2-P
Fonte:
ISI
Lingua:
ENG
Soggetto:
FOLLICULAR-FLUID; FACTOR SYSTEM; DOMINANT FOLLICLE; HORMONE; ATRESIA; PROGESTERONE; MATURATION; EXPRESSION; OVULATION; CATTLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Sirotkin, AV Res Inst Anim Prod, Lab Endocrinol, Hlohovska 2, Nitra 94992,Slovakia Res Inst Anim Prod Hlohovska 2 Nitra Slovakia 94992 Slovakia
Citazione:
A.V. Sirotkin et al., "Secretory activity of bovine ovarian granulosa cells transfected with sense and antisense insulin-like growth factor (IGF) binding protein-3 and the response to IGF-I, GH, LH, oxytocin and oestradiol", J MOL ENDOC, 27(3), 2001, pp. 329-338

Abstract

The aim Of Our in vitro experiments was to examine if IGF binding protein (IGFBP)-3 is involved in control of bovine ovarian secretory activity. For this purpose we performed the transfection of bovine granulosa cells with cDNA sense and antisense constructs increasing or inhibiting IGFBP-3 synthesis. The release of IGFBP-3, progesterone, oxytocin, IGF-I and prostaglandins F (PGF) and E (PGE) by control and transfected cells was compared. The transfected ovarian cells were cultured with and without bLH (100 ng/ml), bGH(100 ng/ml), IGF-I (10 ng/ml), oxytocin (10 ng/ml) and oestradiol-17 beta (100 ng/ml). The concentration of IGFBP-3 produced was assessed using ligand and western blotting and secretion of progesterone, oxytocin, IGF-I, PGF and PGE was evaluated using RIA/IRIMA techniques. Transfection of cells with the sense IGFBP-3 cDNA construct resulted in the expected increase in IGFBP-3 release, whereas the antisense IGFBP-3 construct induced the expected reduction in IGFBP-3 output. The granulosa cells transfected to overexpress IGFBP-3 had an increase in IGF-I, PGF and PGE release, and a decrease in basal and hormone- or growth factor-induced accumulation of progesterone and oxytocin. The granulosa cells transfected to have reduced IGFBP-3 expression gave primarily significant opposite findings. The present results suggest the involvement of IGFBP-3 in control of bovineovarian steroid, peptide hormone, growth factor and prostaglandin release. IGFBP-3 is a physiological stimulator of IGF-I and prostaglandin release and an inhibitor of steroid and peptide hormone output.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 00:22:22