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Titolo:
Cytokines regulate membrane adenosine deaminase on human activated lymphocytes
Autore:
Cordero, OJ; Salgado, FJ; Fernandez-Alonso, CM; Herrera, C; Lluis, C; Franco, R; Nogueira, M;
Indirizzi:
Univ Santiago de Compostela, Dept Biochem, Santiago De Compostela 15706, Spain Univ Santiago de Compostela Santiago De Compostela Spain 15706 06, Spain Univ Santiago de Compostela, Dept Mol Biol, Santiago De Compostela 15706, Spain Univ Santiago de Compostela Santiago De Compostela Spain 15706 06, Spain Univ Barcelona, Dept Biochem, Barcelona 08108, Spain Univ Barcelona Barcelona Spain 08108 ept Biochem, Barcelona 08108, Spain Univ Barcelona, Dept Mol Biol, Barcelona 08108, Spain Univ Barcelona Barcelona Spain 08108 pt Mol Biol, Barcelona 08108, Spain
Titolo Testata:
JOURNAL OF LEUKOCYTE BIOLOGY
fascicolo: 6, volume: 70, anno: 2001,
pagine: 920 - 930
SICI:
0741-5400(200112)70:6<920:CRMADO>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
T-CELL ACTIVATION; ADA ISOENZYMES ADA1; COMBINED IMMUNODEFICIENCY; EXPRESSION; CD26; SURFACE; RECEPTORS; ANTIGEN; BINDING; BLOOD;
Keywords:
ectoenzymes; protein translocation pathways; CD26 interleukins; immunoregulatory mechanisms; Brefeldin A;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Nogueira, M Univ Santiago de Compostela, Fac Biol, Dept Bioquim & Biol Mol, Campus Sur, Santiago De Compostela 15782, Galicia, Spain Univ Santiago deCompostela Campus Sur Santiago De Compostela Galicia Spain 15782
Citazione:
O.J. Cordero et al., "Cytokines regulate membrane adenosine deaminase on human activated lymphocytes", J LEUK BIOL, 70(6), 2001, pp. 920-930

Abstract

CD26 is a lymphocyte marker that can anchor adenosine deaminase (ADA) on the T cell surface. We found that ADA is regulated by cytokines on the cell surface during T cell activation. By means of flow cytometry, immunofluorescence, and immunoblotting techniques, we found that interleukin (IL)-2 and IL-12 up-regulate ecto-ADA and CD26 expression. In clear contrast, IL-4 ledto down-regulation of lymphocyte surface ADA without modifying the level of CD26. Moreover, neither circulating ADA transcription nor mRNA translation was regulated by cytokines. These results, along with absence of total-ADA modulation, the variable amount of ADA found in purified plasma membranes, and the different effect of Brefeldin A on the surface presence of ADA and CD26 indicated that cytokines regulate the translocation of ADA towards the cell surface through a mechanism not involving CD26. Ecto-ADA protected activated lymphocytes from the toxic effects of extracellular adenosine. Therefore, this cell surface ADA control might constitute part of the fine immunoregulatory mechanism of adenosine-mediated signaling through purinergicreceptors in leukocytes.

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Documento generato il 02/12/20 alle ore 14:56:17